Difference between revisions of "CdaR"

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(Biological materials)
(Biological materials)
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* '''Strep-tag construct:'''
 
* '''Strep-tag construct:'''
 
** GP1330 (aphA3, based on [[pGP1460]]), available in [[Jörg Stülke]]'s lab  
 
** GP1330 (aphA3, based on [[pGP1460]]), available in [[Jörg Stülke]]'s lab  
** GP1380 lacA::cdaR-Strep aphA3 glmM-3xFLAG ermC (based on [[pGP1460]] and [[pGP1087]]), available in [[Jörg Stülke]]'s lab  
+
** GP1380 ''lacA::cdaR-Strep aphA3 glmM-3xFLAG ermC'' (based on [[pGP1460]] and [[pGP1087]]), available in [[Jörg Stülke]]'s lab  
  
 
* '''Antibody:'''
 
* '''Antibody:'''

Revision as of 13:44, 18 December 2012

  • Description: effector protein controlling CdaA diadenylate cyclase activity

Gene name cdaR
Synonyms ybbR
Essential no
Product effector protein controlling CdaA diadenylate cyclase activity
Function regulation of c-di-AMP synthesis
Gene expression levels in SubtiExpress: cdaR
Interactions involving this protein in SubtInteract: CdaR
Metabolic function and regulation of this protein in SubtiPathways:
Murein recycling
MW, pI 52 kDa, 4.719
Gene length, protein length 1449 bp, 483 aa
Immediate neighbours cdaA, glmM
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YbbR context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YbbR expression.png















Categories containing this gene/protein

metabolism of signalling nucleotides

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU01760

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • stimulates CdaA activity upon protein-protein interaction PubMed
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information: the cdaA-cdaR part of the mRNA is very stable (half-life > 15 min) PubMed

Biological materials

  • Expression vector:
    • IPTG inducible expression of Strep-cdaR in E. coli: pGP2565 (in pGP172), available in Jörg Stülke's lab
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab. Respective plasmids: pGP1989 and pGP1992.
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Additional publications: PubMed