ccpB

168

transcriptional regulator ([wiki|LacI family])

locus: BSU_40870

Molecular weight: 34.69 kDa

pI: 6.33

Protein length: 311 aa

Gene length: 936 bp

function: unknown

product: transcriptional regulator ([wiki|LacI family])

essential: no

synonyms: ccpB, yyaG

List of homologs in different organisms, belongs to COG1609 (Galperin et al., 2021)

Gene

Coordinates: 4,196,786 → 4,197,721

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The protein

Protein family

• [wiki|LacI family]

[wiki|Domains]

• [wiki|HTH lacI-type domain] (aa 1-56) (according to UniProt)

Structure

• [PDB|2HSG] (B. megaterium [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA], 30% identity) [pubmed|17500051]
• [AF|P37517]

Biological materials

Mutant

• MGNA-B867 (yyaG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1866 NBRP B. subtilis, Japan]
• BKE40870 (Δ[gene|C14B113B1A8BEA0547459AA7EC5755634D1A8A88|ccpB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE40870 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCTCTTTCTCCTTTTCAT,  downstream forward: _UP4_TGAACTGAAATGCATTTCAT
• BKK40870 (Δ[gene|C14B113B1A8BEA0547459AA7EC5755634D1A8A88|ccpB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK40870 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCTCTTTCTCCTTTTCAT,  downstream forward: _UP4_TGAACTGAAATGCATTTCAT

References

• 9457849,16237020,9457849,17500051