ccpA

ccpA
168

Carbon catabolite control protein A, involved in glucose regulation of many genes; represses catabolic genes and activates genes involved in excretion of excess carbon

locus
BSU_29740
Molecular weight
36.78 kDa
pI
5.06
Protein length
Gene length
function
carbon catabolite repression (CCR)
product
transcriptional regulator ([wiki|LacI family])
essential
no
synonyms
ccpA, graR, alsA, amyR

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1609 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,044,165 → 3,045,169
Phenotypes of a mutant
Loss of carbon catabolite repression. Loss of [protein|14ED1AF5038F43F3B151FCBABE6CFC5A2DA3AA6E|ptsI]-dependent sugar transport due to excessive phosphorylation of [protein|29B793660E4D30C0656248F3EF403FEF76FB9025|HPr] by [protein|771F205F818A755A661B8E1C95365A4F6AEB05C7|hprK]
The mutant is unable to grow on a minimal medium with glucose and ammonium as the only sources of carbon and nitrogen, respectively, this can be suppressed by mutations resulting in hyperactive [protein|41199C76DF8CA804B7B8878D61228B9542A96AE4|topoisomerase I] or in the inactivation of [gene|56CBEBCFEF5CFB4A0175498338C7AF2F45EAA3E3|rocG] [pubmed|29242163,17183217]
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The protein
Catalyzed reaction/ biological activity
transcriptional regulator of carbon catabolite repression (CCR)
Protein family
[wiki|LacI family]
[wiki|Domains]
[wiki|HTH lacI-type domain] (aa 1-58) (according to UniProt)
DNA binding Domain  (6 – 25)
[wiki|Cofactors]
[protein|29B793660E4D30C0656248F3EF403FEF76FB9025|HPr]-Ser46-P, [protein|A269774F2FDC94F93BA5F1360FFFE754B50383AD|crh]-Ser-46-P
Structure
[PDB|3OQM] (complex of ''B. subtilis'' CcpA with P-Ser-[protein|29B793660E4D30C0656248F3EF403FEF76FB9025|HPr] and the ''[gene|DAA285C86692E8B47D48652E0973AE3FF091CBC3|ackA]'' operator site)
[PDB|3OQN] (complex of ''B. subtilis'' CcpA with P-Ser-[protein|29B793660E4D30C0656248F3EF403FEF76FB9025|HPr] and the ''[gene|1C566E54F8EF1A17A365FC49DFDA452AC5D0CA64|gntR]'' operator site)
[PDB|3OQO] (complex of ''B. subtilis'' CcpA with P-Ser-[protein|29B793660E4D30C0656248F3EF403FEF76FB9025|HPr] and a optimal synthetic operator site)
[PDB|2HSG] (apoprotein) [pubmed|17500051]
CcpA-[protein|A269774F2FDC94F93BA5F1360FFFE754B50383AD|crh]-DNA-complex [http://www.ncbi.nlm.nih.gov/Structure/mmdb/mmdbsrv.cgi?Dopt=s&uid=52326 NCBI]
complex with P-Ser-[protein|29B793660E4D30C0656248F3EF403FEF76FB9025|HPr] and sulphate ions [http://www.ncbi.nlm.nih.gov/Structure/mmdb/mmdbsrv.cgi?Dopt=s&uid=39857 NCBI]
[AF|P25144]
Effectors of protein activity
glucose-6-phosphate, fructose-1,6-bisphosphate [Pubmed|17376479]
Expression and Regulation
Operons
genes
[gene|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]-[gene|3D42584D65D80A1C73F057714647E29FF6BBD58A|motP]-[gene|6617D785D08C5919F0B774D2AF9EA6A84215486B|motS]
description
[Pubmed|16547058]
regulation
constitutive
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
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[gene|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]→[gene|6617D785D08C5919F0B774D2AF9EA6A84215486B|motS]

2024-03-13 23:00:42

Jstuelk

77

68ef3ce524e54396f2c6cf16cb7c57ee0668374c

6324F2439C0162775E97BE501BAE7401FB6612D4

additional information
there are about 3.000 molecules of CcpA per cell [http://www.ncbi.nlm.nih.gov/sites/entrez/8000527 PubMed], this corresponds to a concentration of 3 µM  (according to [PubMed|20408793])
Biological materials
Mutant
QB5407 (ccpA::spc) [Pubmed|10941796], available in [wiki|Jörg Stülke]'s lab
GP302 (erm) [Pubmed|12123463], available in [wiki|Jörg Stülke]'s lab
GP300 (an in frame deletion of ''[gene|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]'') [Pubmed|11557150], available in [wiki|Jörg Stülke]'s lab
WH649 (aphA3), available in [wiki|Gerald Seidel]'s lab
BKE29740 (Δ[gene|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE29740 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCCTAAAACCACTCCTTT,  downstream forward: _UP4_TAAGAAAAACAAAGAGCAAG
BKK29740 (Δ[gene|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK29740 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCCTAAAACCACTCCTTT,  downstream forward: _UP4_TAAGAAAAACAAAGAGCAAG
Expression vectors
pGP643 (N-terminal Strep-tag, purification from ''B. subtilis'', for [wiki|SPINE], in [wiki|pGP380]), available in [wiki|Jörg Stülke]'s lab
pWH940 (C-terminal Strep-tag, purification from ''B. subtilis'', for [wiki|SPINE], in [wiki|pGP382]), available in [wiki|Gerald Seidel]'s lab
Antibody
available in [wiki|Gerald Seidel]'s and in [wiki|Jörg Stülke]'s lab
labs
[wiki|Gerald Seidel], Erlangen University, Germany [http://www.biologie.uni-erlangen.de/mibi/index2.html Homepage]
[wiki|Richard Brennan], Houston, Texas, USA [http://www.mdanderson.org/departments/biochem/display.cfm?id=556ef368-6c81-4043-b74f350d41dd06cb&method=displayfull&pn=a8427ebd-d0ff-11d4-80fd00508b603a14 Homepage]
[wiki|Milton H. Saier], University of California at San Diego, USA [http://biology.ucsd.edu/faculty/saier.html Homepage]
[wiki|Yasutaro Fujita], University of Fukuyama, Japan
[wiki|Jörg Stülke], University of Göttingen, Germany [http://wwwuser.gwdg.de/~genmibio/stuelke.html Homepage]
[wiki|Oscar Kuipers], University of Groningen, The Netherlands [http://molgen.biol.rug.nl/molgen/index.php Homepage]
References
Reviews
20408793,8598282,19202299,14665673,18628769,18359269
Structural analyses
15369672,16316990,17376479,16755587,17500051,17401189,10666630
CcpA-DNA interaction
8596444,10666464,15885105,7665492,9254709,21106498
Control of CcpA activity
7623661,9973552,9334231,12051938,9689125
Functional analysis of CcpA
10383986,10601226,11557150,9252590,9988473
General and physiological studies
1904524,10941796,12123463,8000527,18757537,16547058,14523131,22001508,22512862,29242163,30096425,36372802
Repression of target genes by CcpA
15150224,16166551,11929549,7913927,17827291,11985717,12100558,7592486,16825793,16491025,21398533,26712933,28974613,34096591,35881471
Positive regulation of gene expression by CcpA
8226682,12193635,10559153,15916605,9811655,10986270,25157083
Global analyses (proteome, transcriptome, ChIP-chip)
12850135,11251851,10559165,11160890,17183215,22383848,22900538,26483775

E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9

Page visits: 14866

Time of last update: 2024-03-28 08:29:59

Author of last update: Jstuelk