CapE

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  • Description: stimulates poly-gamma-glutamate production in the presence of zinc, also involved in extra-chromosomal DNA maintenance

Gene name capE
Synonyms ywtC, pgsE, edmS
Essential no
Product unknown
Function capsule synthesis
Gene expression levels in SubtiExpress: capE
MW, pI 6 kDa, 6.487
Gene length, protein length 165 bp, 55 aa
Immediate neighbours pgdS, capA
Sequences Protein DNA DNA_with_flanks
Genetic context
YwtC context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
CapE expression.png















Categories containing this gene/protein

capsule biosynthesis and degradation, membrane proteins

This gene is a member of the following regulons

DegU regulon

The gene

Basic information

  • Locus tag: BSU35870

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • stimulates poly-gamma-glutamate production in the presence of zinc PubMed
    • also involved in extra-chromosomal DNA maintenance PubMed
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains: N-terminal membrane-anchoring domain and a C-terminal assembly accelerator-like structure PubMed
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Additional information: The operon is not expressed in the lab strain 168 due to lack of expression of DegQ resulting in inactivity of DegS and DegU PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Thomas Candela, Agnès Fouet
Poly-gamma-glutamate in bacteria.
Mol Microbiol: 2006, 60(5);1091-8
[PubMed:16689787] [WorldCat.org] [DOI] (P p)

Original publications

Makoto Ashiuchi, Daisuke Yamashiro, Kento Yamamoto
Bacillus subtilis EdmS (formerly PgsE) participates in the maintenance of episomes.
Plasmid: 2013, 70(2);209-15
[PubMed:23583563] [WorldCat.org] [DOI] (I p)

Thi-Huyen Do, Yuki Suzuki, Naoki Abe, Jun Kaneko, Yoshifumi Itoh, Keitarou Kimura
Mutations suppressing the loss of DegQ function in Bacillus subtilis (natto) poly-γ-glutamate synthesis.
Appl Environ Microbiol: 2011, 77(23);8249-58
[PubMed:21965392] [WorldCat.org] [DOI] (I p)

Daisuke Yamashiro, Yutaka Minouchi, Makoto Ashiuchi
Moonlighting role of a poly-gamma-glutamate synthetase component from Bacillus subtilis: insight into novel extrachromosomal DNA maintenance.
Appl Environ Microbiol: 2011, 77(8);2796-8
[PubMed:21357437] [WorldCat.org] [DOI] (I p)

Daisuke Yamashiro, Megumi Yoshioka, Makoto Ashiuchi
Bacillus subtilis pgsE (Formerly ywtC) stimulates poly-γ-glutamate production in the presence of zinc.
Biotechnol Bioeng: 2011, 108(1);226-30
[PubMed:20812257] [WorldCat.org] [DOI] (I p)

Tohru Kamei, Daisuke Yamashiro, Terumi Horiuchii, Yutaka Minouchi, Makoto Ashiuchi
Identification and biochemical characterization of membranous short-chain polyglutamate from Bacillus subtilis.
Chem Biodivers: 2010, 7(6);1563-72
[PubMed:20564574] [WorldCat.org] [DOI] (I p)

Chuan-Mei Yeh, Jyh-Perng Wang, Shih-Ching Lo, Wen-Chia Chan, Ming-Yi Lin
Chromosomal integration of a synthetic expression control sequence achieves poly-gamma-glutamate production in a Bacillus subtilis strain.
Biotechnol Prog: 2010, 26(4);1001-7
[PubMed:20564357] [WorldCat.org] [DOI] (I p)

Keitarou Kimura, Lam-Son Phan Tran, Thi-Huyen Do, Yoshifumi Itoh
Expression of the pgsB encoding the poly-gamma-DL-glutamate synthetase of Bacillus subtilis (natto).
Biosci Biotechnol Biochem: 2009, 73(5);1149-55
[PubMed:19420703] [WorldCat.org] [DOI] (I p)

Thomas Candela, Michèle Mock, Agnès Fouet
CapE, a 47-amino-acid peptide, is necessary for Bacillus anthracis polyglutamate capsule synthesis.
J Bacteriol: 2005, 187(22);7765-72
[PubMed:16267300] [WorldCat.org] [DOI] (P p)

Yuji Urushibata, Shinji Tokuyama, Yasutaka Tahara
Difference in transcription levels of cap genes for gamma-polyglutamic acid production between Bacillus subtilis IFO 16449 and Marburg 168.
J Biosci Bioeng: 2002, 93(2);252-4
[PubMed:16233197] [WorldCat.org] [DOI] (P p)