cah

168

cephalosporin C deacetylase

locus: BSU_03180

Molecular weight: 35.65 kDa

pI: 5.42

Protein length: 318 aa

Gene length: 957 bp

function: resistance to cephalosporin C

product: cephalosporin C deacetylase)

essential: no

ec: 3.1.1.41

synonyms: cah

List of homologs in different organisms, belongs to COG3458 (Galperin et al., 2021)

Gene

Coordinates: 342,538  343,494

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The protein

Catalyzed reaction/ biological activity

• cephalosporin C + H₂O --> acetate + deacetylcephalosporin C + H⁺ (according to UniProt)

Protein family

• carbohydrate esterase 7 family (single member, according to UniProt)

Structure

• [PDB|1ODS] [pubmed|12842474]
• [AF|P94388]

[wiki|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|6BCE78F623A40B519211E79AFA1AD898318BD817|cah]

  description: [Pubmed|7793942]

• genes: [gene|D28190620D5B834DD7F42822B835C6FE932FD8A5|ycgK]-[gene|6BCE78F623A40B519211E79AFA1AD898318BD817|cah]-[gene|7306A55192AEB4032FD17A1BAAFF1127D511D70E|ycgL]

  description: [Pubmed|22383849]

Biological materials

Mutant

• BKE03180 ([gene|6BCE78F623A40B519211E79AFA1AD898318BD817|cah]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE03180 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTCCGATTATTCCCCTT,  downstream forward: _UP4_TGATAAATGTGAAAAGCCGC
• BKK03180 ([gene|6BCE78F623A40B519211E79AFA1AD898318BD817|cah]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK03180 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTCCGATTATTCCCCTT,  downstream forward: _UP4_TGATAAATGTGAAAAGCCGC

References

• 12842474,7793942,15069587,16232499,12842474