Difference between revisions of "CadA"

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|style="background:#ABCDEF;" align="center"|'''Function''' || cadmium export
 
|style="background:#ABCDEF;" align="center"|'''Function''' || cadmium export
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU33490 cadA]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU33490 cadA]
 
|-
 
|-
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://cellpublisher.gobics.de/view/GqbblRJkBH metal ion homeostasis]'''
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://cellpublisher.gobics.de/view/GqbblRJkBH metal ion homeostasis]'''
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[bdbD]]'', ''[[copA]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[bdbD]]'', ''[[copA]]''
 
|-
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB15354&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU33490 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU33490 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU33490 Advanced_DNA]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:yvgW_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:yvgW_context.gif]]

Revision as of 13:50, 13 May 2013

  • Description: cadmium transporting ATPase, resistance to cadmium

Gene name cadA
Synonyms yvgW
Essential no
Product cadmium transporting ATPase
Function cadmium export
Gene expression levels in SubtiExpress: cadA
Metabolic function and regulation of this protein in SubtiPathways:
metal ion homeostasis
MW, pI 75 kDa, 5.998
Gene length, protein length 2106 bp, 702 aa
Immediate neighbours bdbD, copA
Sequences Protein DNA Advanced_DNA
Genetic context
YvgW context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
CadA expression.png
























Categories containing this gene/protein

transporters/ other, resistance against toxic metals, membrane proteins

This gene is a member of the following regulons

CzrA regulon

The gene

Basic information

  • Locus tag: BSU33490

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + H2O + Cd2+(In) = ADP + phosphate + Cd2+(Out) (according to Swiss-Prot)
  • Protein family: Type IB subfamily (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]

Additional information

Expression and regulation

  • Regulation:
    • induced by toxic metal ions (Zn(II), Cd(II), Co(II), Ni(II) and Cu(II)) (CzrA) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

John Helmann, Cornell University, USA Homepage

Your additional remarks

References

Charles M Moore, Ahmed Gaballa, Monica Hui, Rick W Ye, John D Helmann
Genetic and physiological responses of Bacillus subtilis to metal ion stress.
Mol Microbiol: 2005, 57(1);27-40
[PubMed:15948947] [WorldCat.org] [DOI] (P p)

Ahmed Gaballa, John D Helmann
Bacillus subtilis CPx-type ATPases: characterization of Cd, Zn, Co and Cu efflux systems.
Biometals: 2003, 16(4);497-505
[PubMed:12779235] [WorldCat.org] [DOI] (P p)

I M Solovieva, K D Entian
Investigation of the yvgW Bacillus subtilis chromosomal gene involved in Cd(2+) ion resistance.
FEMS Microbiol Lett: 2002, 208(1);105-9
[PubMed:11934502] [WorldCat.org] [DOI] (P p)