buk

buk
168

butyrate kinase

locus
BSU_24070
Molecular weight
39.61 kDa
pI
5.47
Protein length
Gene length
function
utilization of branched-chain keto acids
product
butyrate kinase
essential
no
ec
2.7.2.7
synonyms
buk, yqiU, bkd

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG3426 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,501,549  2,502,640
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The protein
Catalyzed reaction/ biological activity
ATP + butanoate --> ADP + butanoyl phosphate (according to UniProt)
Protein family
acetokinase family (with [protein|DAA285C86692E8B47D48652E0973AE3FF091CBC3|ackA], according to UniProt)
Structure
[PDB|1X9J] (from ''Thermotoga maritima'', 52% identity, 69% similarity)
[AF|P54532]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|1CFF7202AC6078B6BAD1253F77FD4FE227C046DB|ptb]-[gene|A52E50104B18D0A8518218C52D9CC36FDDB29AAF|bcd]-[gene|8E08A0333E4EC89003DA3C1DD8DCD39CDDC8624C|buk]-[gene|1FD06CB6E81C920EFC656DBE2A13D68B1AA68872|lpdV]-[gene|9F298088C0A9EB7FE140C935AFC9243C6D4DE8AE|bkdAA]-[gene|A024921961A786294199EA12E04456C890ED8D8C|bkdAB]-[gene|262C9FD20C7A70B6F1FEB57735FA800F38EAB25A|bkdB]
description
[Pubmed|12823818]
regulation
induced in the presence of isoleucine or valine ([protein|search|BkdR]) [Pubmed|10094682]
regulatory mechanism
[protein|4CEBD81F485DD0B660E297FFC34A0F5270652184|bkdR]: activation, [Pubmed|10094682], in [regulon|protein:4CEBD81F485DD0B660E297FFC34A0F5270652184|bkdR regulon]
[protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY]: repression, [Pubmed|10094682], in [regulon|protein:90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY regulon]
sigma factors
[protein|1118A21CC581B0470EC6E7C178F2523CDCA70F93|sigL]: sigma factor, [Pubmed|10094682], in [regulon|protein:1118A21CC581B0470EC6E7C178F2523CDCA70F93|sigL regulon]
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[gene|1CFF7202AC6078B6BAD1253F77FD4FE227C046DB|ptb]→[gene|262C9FD20C7A70B6F1FEB57735FA800F38EAB25A|bkdB]

2024-02-26 13:35:02

ghost

56

7b8921dc3f8fdb775067a1f92187895088ac1126

0BF3F61A9229E387986A64E3D2F9F647D24EA48A

Biological materials
Mutant
MGNA-C378 (yqiU::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2376 NBRP B. subtilis, Japan]
BKE24070 ([gene|8E08A0333E4EC89003DA3C1DD8DCD39CDDC8624C|buk]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE24070 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TTTCATAACCTCCAGATCAA,  downstream forward: _UP4_TAGACAGACAGGAGTGAGTC
BKK24070 ([gene|8E08A0333E4EC89003DA3C1DD8DCD39CDDC8624C|buk]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK24070 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TTTCATAACCTCCAGATCAA,  downstream forward: _UP4_TAGACAGACAGGAGTGAGTC
References
36815589,12427936,15241682,10094682,12823818

8E08A0333E4EC89003DA3C1DD8DCD39CDDC8624C

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Time of last update: 2024-03-28 20:42:32

Author of last update: Jstuelk