Difference between revisions of "BsrF"

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(Labs working on this gene/RNA)
(Biological materials)
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=Biological materials =
 
=Biological materials =
  
* '''Mutant:'''
+
* '''Mutants:''' DB104::Delta bsrF::cat, DB104::Delta bsrF::ery, 168::Delta bsrF::cat, 168::Delta bsrF::ery
  
* '''Expression vector:'''
+
* '''Expression vectors:''' pWSR2, multi-copy vector for tetracycline-inducible expression of bsrF (kanamycin resistance gene)
 +
  pUCB2SR2, multi-copy vector for constitutive expression of bsrF under its own promoter (kanamycin resistance gene)
 
          
 
          
* '''lacZ fusion:''' available in the [[Sabine Brantl]] lab
+
* '''lacZ fusions:''' pACG1 to pACG19 containing regions of different size upstream of the bsrF promoter available in the [[Sabine Brantl]] lab
  
 
=Labs working on this gene/RNA=
 
=Labs working on this gene/RNA=

Revision as of 16:56, 14 July 2009

  • Description: small RNA

Gene name bsrF
Synonyms SR2
Essential no
Product small RNA
Function unknown
Gene length bp
Immediate neighbours yobO, csaA
Gene sequence (+200bp)
Genetic context
File:GenE context.gif








The gene

Basic information

  • Locus tag:

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: no entry
  • Structure:

Additional information

Expression and regulation

  • Regulatory mechanism: CodY: transcription activation PubMed
  • Additional information:

Biological materials

  • Mutants: DB104::Delta bsrF::cat, DB104::Delta bsrF::ery, 168::Delta bsrF::cat, 168::Delta bsrF::ery
  • Expression vectors: pWSR2, multi-copy vector for tetracycline-inducible expression of bsrF (kanamycin resistance gene)
 pUCB2SR2, multi-copy vector for constitutive expression of bsrF under its own promoter (kanamycin resistance gene)
       
  • lacZ fusions: pACG1 to pACG19 containing regions of different size upstream of the bsrF promoter available in the Sabine Brantl lab

Labs working on this gene/RNA

  • Sabine Brantl, Bacterial Genetics, Friedrich-Schiller-University of Jena, Germany homepage
  • Kouji Nakamura, Tsukuba, University, Japan

Your additional remarks

References

Heike Preis, Rita A Eckart, Rajani K Gudipati, Nadja Heidrich, Sabine Brantl
CodY activates transcription of a small RNA in Bacillus subtilis.
J Bacteriol: 2009, 191(17);5446-57
[PubMed:19542274] [WorldCat.org] [DOI] (I p)

Shinichi Saito, Hiroshi Kakeshita, Kouji Nakamura
Novel small RNA-encoding genes in the intergenic regions of Bacillus subtilis.
Gene: 2009, 428(1-2);2-8
[PubMed:18948176] [WorldCat.org] [DOI] (P p)