Difference between revisions of "BslA"

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m (moved YuaB to BslA: 22571672)
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* '''Description:''' required for complex colony architecture, facilitates biofilm matrix assembly<br/><br/>
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* '''Description:''' amphiphilic protein, forms water-repellent surface layer of the biofilm<br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
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|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
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|style="background:#ABCDEF;" align="center"| '''Product''' || cell wall protein
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|style="background:#ABCDEF;" align="center"| '''Product''' || biofilm surface layer
 
|-
 
|-
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|style="background:#ABCDEF;" align="center"|'''Function''' || pellicle formation
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|style="background:#ABCDEF;" align="center"|'''Function''' || [[biofilm formation]]
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 19 kDa, 9.987   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 19 kDa, 9.987   
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===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
 
* reduced colony complexity {{PubMed|21742882}}
 
* reduced colony complexity {{PubMed|21742882}}
 +
* loss of surface repellency of the biofilm {{PubMed|22571672}}
  
 
=== Database entries ===
 
=== Database entries ===
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* '''Protein family:'''
 
* '''Protein family:'''
  
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* '''Paralogous protein(s):'''
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* '''Paralogous protein(s):''' [[YweA]]
  
 
=== Extended information on the protein ===
 
=== Extended information on the protein ===
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* '''Localization:'''  
 
* '''Localization:'''  
 
** extracellular (signal peptide) [http://www.ncbi.nlm.nih.gov/pubmed/18957862 PubMed]
 
** extracellular (signal peptide) [http://www.ncbi.nlm.nih.gov/pubmed/18957862 PubMed]
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** cell wall {{PubMed|21742882}}
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** biofilm matrix in an extracellular polysaccharide-dependent manner {{PubMed|22571672}}
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** forms a layer on the biofilm surface {{PubMed|22571672}}
  
 
=== Database entries ===
 
=== Database entries ===
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=Expression and regulation=
 
=Expression and regulation=
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* '''Operon:'''  
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* '''Operon:''' ''[[bslA]]'' {{PubMed|22383849}}
  
 
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=yuaB_3187503_3188048_1 yuaB] {{PubMed|22383849}}
 
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=yuaB_3187503_3188048_1 yuaB] {{PubMed|22383849}}
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=References=
 
=References=
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<pubmed> 18978066 18957862, 17850253 21097620 21742882 </pubmed>
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<pubmed> 18978066 18957862, 17850253 21097620 21742882 22383849 22571672 </pubmed>
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 11:26, 11 May 2012

  • Description: amphiphilic protein, forms water-repellent surface layer of the biofilm

Gene name yuaB
Synonyms
Essential no
Product biofilm surface layer
Function biofilm formation
MW, pI 19 kDa, 9.987
Gene length, protein length 543 bp, 181 aa
Immediate neighbours gbsR, ktrA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YuaB context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YuaB expression.png
























Categories containing this gene/protein

biofilm formation

This gene is a member of the following regulons

AbrB regulon, DegU regulon

The gene

Basic information

  • Locus tag: BSU31080

Phenotypes of a mutant

  • reduced colony complexity PubMed
  • loss of surface repellency of the biofilm PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s): YweA

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:
    • extracellular (signal peptide) PubMed
    • biofilm matrix in an extracellular polysaccharide-dependent manner PubMed
    • forms a layer on the biofilm surface PubMed

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
    • expression is down-regulated in a rok mutant (indirect effect) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Kazuo Kobayashi, Megumi Iwano
BslA(YuaB) forms a hydrophobic layer on the surface of Bacillus subtilis biofilms.
Mol Microbiol: 2012, 85(1);51-66
[PubMed:22571672] [WorldCat.org] [DOI] (I p)

Pierre Nicolas, Ulrike Mäder, Etienne Dervyn, Tatiana Rochat, Aurélie Leduc, Nathalie Pigeonneau, Elena Bidnenko, Elodie Marchadier, Mark Hoebeke, Stéphane Aymerich, Dörte Becher, Paola Bisicchia, Eric Botella, Olivier Delumeau, Geoff Doherty, Emma L Denham, Mark J Fogg, Vincent Fromion, Anne Goelzer, Annette Hansen, Elisabeth Härtig, Colin R Harwood, Georg Homuth, Hanne Jarmer, Matthieu Jules, Edda Klipp, Ludovic Le Chat, François Lecointe, Peter Lewis, Wolfram Liebermeister, Anika March, Ruben A T Mars, Priyanka Nannapaneni, David Noone, Susanne Pohl, Bernd Rinn, Frank Rügheimer, Praveen K Sappa, Franck Samson, Marc Schaffer, Benno Schwikowski, Leif Steil, Jörg Stülke, Thomas Wiegert, Kevin M Devine, Anthony J Wilkinson, Jan Maarten van Dijl, Michael Hecker, Uwe Völker, Philippe Bessières, Philippe Noirot
Condition-dependent transcriptome reveals high-level regulatory architecture in Bacillus subtilis.
Science: 2012, 335(6072);1103-6
[PubMed:22383849] [WorldCat.org] [DOI] (I p)

Adam Ostrowski, Angela Mehert, Alan Prescott, Taryn B Kiley, Nicola R Stanley-Wall
YuaB functions synergistically with the exopolysaccharide and TasA amyloid fibers to allow biofilm formation by Bacillus subtilis.
J Bacteriol: 2011, 193(18);4821-31
[PubMed:21742882] [WorldCat.org] [DOI] (I p)

Akos T Kovács, Oscar P Kuipers
Rok regulates yuaB expression during architecturally complex colony development of Bacillus subtilis 168.
J Bacteriol: 2011, 193(4);998-1002
[PubMed:21097620] [WorldCat.org] [DOI] (I p)

Daniel T Verhamme, Ewan J Murray, Nicola R Stanley-Wall
DegU and Spo0A jointly control transcription of two loci required for complex colony development by Bacillus subtilis.
J Bacteriol: 2009, 191(1);100-8
[PubMed:18978066] [WorldCat.org] [DOI] (I p)

Birgit Voigt, Haike Antelmann, Dirk Albrecht, Armin Ehrenreich, Karl-Heinz Maurer, Stefan Evers, Gerhard Gottschalk, Jan Maarten van Dijl, Thomas Schweder, Michael Hecker
Cell physiology and protein secretion of Bacillus licheniformis compared to Bacillus subtilis.
J Mol Microbiol Biotechnol: 2009, 16(1-2);53-68
[PubMed:18957862] [WorldCat.org] [DOI] (I p)

Kazuo Kobayashi
Gradual activation of the response regulator DegU controls serial expression of genes for flagellum formation and biofilm formation in Bacillus subtilis.
Mol Microbiol: 2007, 66(2);395-409
[PubMed:17850253] [WorldCat.org] [DOI] (P p)

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