Difference between revisions of "BshC"

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=== Additional information===
 
=== Additional information===
 
 
 
  
 
=The protein=
 
=The protein=
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* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU15120&redirect=T BSU15120]
 
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU15120&redirect=T BSU15120]
  
* '''Structure:'''
+
* '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?structureId=4wbd 4WBD] {{PubMed|25496067}}
  
 
* '''UniProt:''' [http://www.uniprot.org/uniprot/P55342 P55342]
 
* '''UniProt:''' [http://www.uniprot.org/uniprot/P55342 P55342]
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* '''[[Sigma factor]]:'''
 
* '''[[Sigma factor]]:'''
** ''[[ylbQ]]'': [[SigA]] {{PubMed|23894131}}  
+
** ''[[ylbQ]]'': [[SigA]] {{PubMed|23894131}}  
** ''[[bshC]]'': [[SigA]] {{PubMed|23894131}}  
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** ''[[bshC]]'': [[SigA]] {{PubMed|23894131}}  
  
 
* '''Regulation:'''  
 
* '''Regulation:'''  
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=References=
 
=References=
<pubmed> 8636036 20308541 19578333 23894131</pubmed>
+
<pubmed>25496067 8636036 20308541 19578333 23894131</pubmed>
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 17:25, 16 February 2015

  • Description: cysteine-adding enzyme required for the synthesis of bacillithiol

Gene name bshC
Synonyms yllA
Essential no
Product cysteine-adding enzyme
Function biosynthesis of bacillithiol
Gene expression levels in SubtiExpress: bshC
MW, pI 62 kDa, 5.247
Gene length, protein length 1617 bp, 539 aa
Immediate neighbours ylbQ, mraZ
Sequences Protein DNA DNA_with_flanks
Genetic context
YllA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
BshC expression.png















Categories containing this gene/protein

miscellaneous metabolic pathways, resistance against oxidative and electrophile stress

This gene is a member of the following regulons

Spx regulon


The gene

Basic information

  • Locus tag: BSU15120

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 212 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 717 PubMed

Biological materials

  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Andrew J VanDuinen, Kelsey R Winchell, Mary E Keithly, Paul D Cook
X-ray crystallographic structure of BshC, a unique enzyme involved in bacillithiol biosynthesis.
Biochemistry: 2015, 54(2);100-3
[PubMed:25496067] [WorldCat.org] [DOI] (I p)

Ahmed Gaballa, Haike Antelmann, Chris J Hamilton, John D Helmann
Regulation of Bacillus subtilis bacillithiol biosynthesis operons by Spx.
Microbiology (Reading): 2013, 159(Pt 10);2025-2035
[PubMed:23894131] [WorldCat.org] [DOI] (I p)

Ahmed Gaballa, Gerald L Newton, Haike Antelmann, Derek Parsonage, Heather Upton, Mamta Rawat, Al Claiborne, Robert C Fahey, John D Helmann
Biosynthesis and functions of bacillithiol, a major low-molecular-weight thiol in Bacilli.
Proc Natl Acad Sci U S A: 2010, 107(14);6482-6
[PubMed:20308541] [WorldCat.org] [DOI] (I p)

Gerald L Newton, Mamta Rawat, James J La Clair, Vishnu Karthik Jothivasan, Tanya Budiarto, Chris J Hamilton, Al Claiborne, John D Helmann, Robert C Fahey
Bacillithiol is an antioxidant thiol produced in Bacilli.
Nat Chem Biol: 2009, 5(9);625-7
[PubMed:19578333] [WorldCat.org] [DOI] (I p)

R A Daniel, A M Williams, J Errington
A complex four-gene operon containing essential cell division gene pbpB in Bacillus subtilis.
J Bacteriol: 1996, 178(8);2343-50
[PubMed:8636036] [WorldCat.org] [DOI] (P p)