BshB1

From SubtiWiki
Revision as of 12:07, 17 December 2013 by Jstuelk (talk | contribs)
Jump to: navigation, search
  • Description: N-acetylglucosamin-malate deacetylase, involved in bacillithiol synthesis (major enzyme)

Gene name bshB1
Synonyms jojG, ypjG
Essential no
Product N-acetylglucosamin-malate deacetylase
Function biosynthesis of bacillithiol
Gene expression levels in SubtiExpress: bshB1
Metabolic function and regulation of this protein in SubtiPathways:
Lys, Thr
MW, pI 24 kDa, 6.343
Gene length, protein length 672 bp, 224 aa
Immediate neighbours bshA, mgsA
Sequences Protein DNA DNA_with_flanks
Genetic context
YpjG context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
BshB1 expression.png















Categories containing this gene/protein

miscellaneous metabolic pathways, resistance against oxidative and electrophile stress

This gene is a member of the following regulons

Spx regulon


The gene

Basic information

  • Locus tag: BSU22470

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: GlcNAc-Mal -> GlcN-Mal + Ac
  • Protein family:
  • Paralogous protein(s): BshB2

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • induced by diamide stess (thiol depletion) (Spx) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Ahmed Gaballa, Haike Antelmann, Chris J Hamilton, John D Helmann
Regulation of Bacillus subtilis bacillithiol biosynthesis operons by Spx.
Microbiology (Reading): 2013, 159(Pt 10);2025-2035
[PubMed:23894131] [WorldCat.org] [DOI] (I p)

Zhong Fang, Alexandra A Roberts, Karissa Weidman, Sunil V Sharma, Al Claiborne, Christopher J Hamilton, Patricia C Dos Santos
Cross-functionalities of Bacillus deacetylases involved in bacillithiol biosynthesis and bacillithiol-S-conjugate detoxification pathways.
Biochem J: 2013, 454(2);239-47
[PubMed:23758290] [WorldCat.org] [DOI] (I p)

Ahmed Gaballa, Gerald L Newton, Haike Antelmann, Derek Parsonage, Heather Upton, Mamta Rawat, Al Claiborne, Robert C Fahey, John D Helmann
Biosynthesis and functions of bacillithiol, a major low-molecular-weight thiol in Bacilli.
Proc Natl Acad Sci U S A: 2010, 107(14);6482-6
[PubMed:20308541] [WorldCat.org] [DOI] (I p)

Gerald L Newton, Mamta Rawat, James J La Clair, Vishnu Karthik Jothivasan, Tanya Budiarto, Chris J Hamilton, Al Claiborne, John D Helmann, Robert C Fahey
Bacillithiol is an antioxidant thiol produced in Bacilli.
Nat Chem Biol: 2009, 5(9);625-7
[PubMed:19578333] [WorldCat.org] [DOI] (I p)