Difference between revisions of "BshB1"

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(Biological materials)
(Biological materials)
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=Biological materials =
 
=Biological materials =
  
* '''Mutant:'''  ''bshB1'' null mutant and ''bshB1''/''bshB2''double bull mutant available in [[John Helmann]] lab  
+
* '''Mutant:'''  ''bshB1'' null mutant and ''bshB1'' / ''bshB2'' double bull mutant available in [[John Helmann]] lab  
  
 
* '''Expression vector:'''
 
* '''Expression vector:'''

Revision as of 15:53, 24 March 2010

  • Description: N-acetyl hydrolase involved in bacillithiol synthesis

Gene name BshB1
Synonyms jojG, ypjG
Essential no
Product unknown
Function unknown
Metabolic function and regulation of this protein in SubtiPathways:
Lys, Thr
MW, pI 24 kDa, 6.343
Gene length, protein length 672 bp, 224 aa
Immediate neighbours ypjH, mgsA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YpjG context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU22470

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: GlcNAc-Mal -> GlcN-Mal + Ac
  • Protein family:
  • Paralogous protein(s): BshB2

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant: bshB1 null mutant and bshB1 / bshB2 double bull mutant available in John Helmann lab
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Ahmed Gaballa, Gerald L Newton, Haike Antelmann, Derek Parsonage, Heather Upton, Mamta Rawat, Al Claiborne, Robert C Fahey, John D Helmann
Biosynthesis and functions of bacillithiol, a major low-molecular-weight thiol in Bacilli.
Proc Natl Acad Sci U S A: 2010, 107(14);6482-6
[PubMed:20308541] [WorldCat.org] [DOI] (I p)