Difference between revisions of "BshA"

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(Biological materials)
(Biological materials)
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=Biological materials =
 
=Biological materials =
  
* '''Mutant:''' ''bshA::mls'' available in Helmann lab [[http://subtiwiki.uni-goettingen.de/wiki/index.php/John_Helmann]]
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* '''Mutant:''' ''bshA::mls'' available in [[John_Helmann]] lab
  
 
* '''Expression vector:'''
 
* '''Expression vector:'''

Revision as of 14:46, 24 March 2010

  • Description: Glycosyltransferase involved in bacillithiol synthesis

Gene name BshA
Synonyms jojH, ypjH
Essential no
Product unknown
Function unknown
MW, pI 41 kDa, 6.149
Gene length, protein length 1131 bp, 377 aa
Immediate neighbours cca, ypjG
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YpjH context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU22460

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: UDP-GlcNAc + L-malate = GlcNAc(α1→2)L-malate [2]
  • Protein family: NamA subfamily (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Ahmed Gaballa, Gerald L Newton, Haike Antelmann, Derek Parsonage, Heather Upton, Mamta Rawat, Al Claiborne, Robert C Fahey, John D Helmann
Biosynthesis and functions of bacillithiol, a major low-molecular-weight thiol in Bacilli.
Proc Natl Acad Sci U S A: 2010, 107(14);6482-6
[PubMed:20308541] [WorldCat.org] [DOI] (I p)