bsdC

168

phenolic acid decarboxylase

locus: BSU_03640

Molecular weight: 52.86 kDa

pI: 5.12

Protein length: 473 aa

Gene length: 1422 bp

function: resistance to salicylic acid

product: phenolic acid decarboxylase

essential: no

synonyms: bsdC, yclC

List of homologs in different organisms, belongs to COG0043 (Galperin et al., 2021)

Gene

Coordinates: 413,157 → 414,578

Advertisement

The protein

Catalyzed reaction/ biological activity

• conversion of vanillic acid to guaiacol [Pubmed|26658822]
• 4-hydroxybenzoate + H⁺ --> CO₂ + phenol (according to UniProt)
• 4-hydroxy-3-methoxybenzoate + H⁺ --> CO₂ + guaiacol (according to UniProt)

Protein family

• ubiD family (single memberm, according to UniProt)

Structure

• [PDB|5M1B] (from E. coli, 31% identity) [pubmed|28057757]
• [AF|P94405]

Biological materials

Mutant

• MGNA-C062 (yclC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2060 NBRP B. subtilis, Japan]
• BKE03640 (Δ[gene|FD23F4C2AFCBFE35A79116C5A707FACD454E4180|bsdC]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE03640 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TCTGAAATCTTGATAAGCCA,  downstream forward: _UP4_AATAAATAAGGAAAGGATGT
• BKK03640 (Δ[gene|FD23F4C2AFCBFE35A79116C5A707FACD454E4180|bsdC]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK03640 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TCTGAAATCTTGATAAGCCA,  downstream forward: _UP4_AATAAATAAGGAAAGGATGT

References

• 15979273,17295427,26658822,28057757