brnQ

168

branched-chain amino acid transporter

locus: BSU_26690

Molecular weight: 46.84 kDa

pI: 9.78

Protein length: 440 aa

Gene length: 1323 bp

function: uptake of valine and isoleucine

product: branched-chain amino acid transporter

essential: no

synonyms: brnQ, yrdJ

List of homologs in different organisms, belongs to COG1114 (Galperin et al., 2021)

Gene

Coordinates: 2,727,160  2,728,482

Phenotypes of a mutant

• no phenotype for the single mutant, the triple [gene|210C83EBEBD3DE4946BC511E0249D0032A7CE3D0|bcaP] [gene|8BB8BDA602CC26B0A9E8C22666F0EC539306CF39|brnQ] [gene|75A874F0C344E8404BE39C32EFF59CE562193640|braB] mutant is strongly impaired in the transport of isoleucine and valine at low concentrations [Pubmed|25645558]

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The protein

Catalyzed reaction/ biological activity

• high affinity uptake of isoleucine and valine [Pubmed|25645558]

Protein family

• branched chain amino acid transporter family (together with [protein|75A874F0C344E8404BE39C32EFF59CE562193640|braB]) (according to UniProt)

Structure

• [AF|P94499]

Paralogous protein(s)

• [protein|75A874F0C344E8404BE39C32EFF59CE562193640|braB]

Biological materials

Mutant

• BKE26690 ([gene|8BB8BDA602CC26B0A9E8C22666F0EC539306CF39|brnQ]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE26690 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTATATATCCTCCGATTA,  downstream forward: _UP4_ATTCCAGCTATTGCAGGAGG
• BKK26690 ([gene|8BB8BDA602CC26B0A9E8C22666F0EC539306CF39|brnQ]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK26690 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTATATATCCTCCGATTA,  downstream forward: _UP4_ATTCCAGCTATTGCAGGAGG

References

• 9287000,25645558