Difference between revisions of "BrnQ"

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= [[Categories]] containing this gene/protein =
 
= [[Categories]] containing this gene/protein =
 
{{SubtiWiki category|[[transporters/ other]]}},
 
{{SubtiWiki category|[[transporters/ other]]}},
{{SubtiWiki category|[[biosynthesis/ acquisition of amino acids]]}},
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{{SubtiWiki category|[[biosynthesis/ acquisition of amino acids]]}}
{{SubtiWiki category|[[utilization of amino acids]]}}
 
  
 
= This gene is a member of the following [[regulons]] =
 
= This gene is a member of the following [[regulons]] =
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===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
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* no phenotype for the single mutant, the triple ''[[bcaP]] [[brnQ]] [[braB]]'' mutant is strongly impaired in the transport of isoleucine and valine at low concentrations {{PubMed|25645558}}
  
 
=== Database entries ===
 
=== Database entries ===
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=== Additional information===
 
=== Additional information===
 
 
  
  
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* '''Catalyzed reaction/ biological activity:'''  
 
* '''Catalyzed reaction/ biological activity:'''  
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** high affinity uptake of isoleucine and valine {{PubMed|25645558}}
  
 
* '''Protein family:''' branched chain amino acid transporter family (according to Swiss-Prot)
 
* '''Protein family:''' branched chain amino acid transporter family (according to Swiss-Prot)
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=References=
 
=References=
<pubmed> 9287000 </pubmed>  
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<pubmed> 9287000 25645558</pubmed>  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 14:47, 4 February 2015

Gene name brnQ
Synonyms yrdJ
Essential no
Product branched-chain amino acid transporter
Function uptake of branched-chain amino acids
Gene expression levels in SubtiExpress: brnQ
Metabolic function and regulation of this protein in SubtiPathways:
brnQ
MW, pI 46 kDa, 9.777
Gene length, protein length 1320 bp, 440 aa
Immediate neighbours yrdK, azlD
Sequences Protein DNA DNA_with_flanks
Genetic context
BrnQ context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
BrnQ expression.png















Categories containing this gene/protein

transporters/ other, biosynthesis/ acquisition of amino acids

This gene is a member of the following regulons

AzlB regulon

The gene

Basic information

  • Locus tag: BSU26690

Phenotypes of a mutant

  • no phenotype for the single mutant, the triple bcaP brnQ braB mutant is strongly impaired in the transport of isoleucine and valine at low concentrations PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • high affinity uptake of isoleucine and valine PubMed
  • Protein family: branched chain amino acid transporter family (according to Swiss-Prot)
  • Paralogous protein(s): BraB

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Sigma factor:
  • Regulatory mechanism: AzlB: transcriptional repression PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Boris R Belitsky
Role of branched-chain amino acid transport in Bacillus subtilis CodY activity.
J Bacteriol: 2015, 197(8);1330-8
[PubMed:25645558] [WorldCat.org] [DOI] (I p)

B R Belitsky, M C Gustafsson, A L Sonenshein, C Von Wachenfeldt
An lrp-like gene of Bacillus subtilis involved in branched-chain amino acid transport.
J Bacteriol: 1997, 179(17);5448-57
[PubMed:9287000] [WorldCat.org] [DOI] (P p)