BkdR

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Gene name bkdR
Synonyms yqiR
Essential no
Product transcriptional activator (for SigL-dependent promoter))
Function regulation of branched-chain amino acid utilization,

required for survival at low temperatures)

Gene expression levels in SubtiExpress: bkdR

required for survival at low temperatures)

Interactions involving this protein in SubtInteract: BkdR
Metabolic function and regulation of this protein in SubtiPathways:
Lipid synthesis
MW, pI 77 kDa, 6.237
Gene length, protein length 2076 bp, 692 aa
Immediate neighbours ptb, yqzF
Sequences Protein DNA DNA_with_flanks
Genetic context
BkdR context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
BkdR expression.png















Categories containing this gene/protein

utilization of amino acids, transcription factors and their control

This gene is a member of the following regulons

CodY regulon

The BkdR regulon:

The gene

Basic information

  • Locus tag: BSU24100

Phenotypes of a mutant

The mutant is cold-sensitive PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
    • repressed during growth in the presence of branched chain amino acids (CodY) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Bjorn A Traag, Antonia Pugliese, Jonathan A Eisen, Richard Losick
Gene conservation among endospore-forming bacteria reveals additional sporulation genes in Bacillus subtilis.
J Bacteriol: 2013, 195(2);253-60
[PubMed:23123912] [WorldCat.org] [DOI] (I p)

Frank Wiegeshoff, Carsten L Beckering, Michel Debarbouille, Mohamed A Marahiel
Sigma L is important for cold shock adaptation of Bacillus subtilis.
J Bacteriol: 2006, 188(8);3130-3
[PubMed:16585774] [WorldCat.org] [DOI] (P p)

Ken-ichi Yoshida, Hirotake Yamaguchi, Masaki Kinehara, Yo-hei Ohki, Yoshiko Nakaura, Yasutaro Fujita
Identification of additional TnrA-regulated genes of Bacillus subtilis associated with a TnrA box.
Mol Microbiol: 2003, 49(1);157-65
[PubMed:12823818] [WorldCat.org] [DOI] (P p)

M Debarbouille, R Gardan, M Arnaud, G Rapoport
Role of bkdR, a transcriptional activator of the sigL-dependent isoleucine and valine degradation pathway in Bacillus subtilis.
J Bacteriol: 1999, 181(7);2059-66
[PubMed:10094682] [WorldCat.org] [DOI] (P p)