bkdR

168

transcriptional activator of the [gene|1CFF7202AC6078B6BAD1253F77FD4FE227C046DB|ptb]-[gene|A52E50104B18D0A8518218C52D9CC36FDDB29AAF|bcd]-[gene|8E08A0333E4EC89003DA3C1DD8DCD39CDDC8624C|buk]-[gene|1FD06CB6E81C920EFC656DBE2A13D68B1AA68872|lpdV]-[gene|9F298088C0A9EB7FE140C935AFC9243C6D4DE8AE|bkdAA]-[gene|A024921961A786294199EA12E04456C890ED8D8C|bkdAB]-[gene|262C9FD20C7A70B6F1FEB57735FA800F38EAB25A|bkdB] operon

locus: BSU_24100

Molecular weight: 77.75 kDa

pI: 6.24

Protein length: 692 aa

Gene length: 2079 bp

function: regulation of branched-chain amino acid utilization

product: transcriptional activator (for [protein|1118A21CC581B0470EC6E7C178F2523CDCA70F93|sigL]-dependent promoter)

essential: no

synonyms: bkdR, yqiR

List of homologs in different organisms, belongs to COG3829 (Galperin et al., 2021)

Gene

Coordinates: 2,504,789  2,506,867

Phenotypes of a mutant

• cold-sensitive [pubmed|16585774]

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The protein

Protein family

• [wiki|Transcription factors activating transcription at SigL-dependent promoters]

[wiki|Domains]

• 2 [wiki|PAS domain]s (aa 115-185, aa 234-305) (according to UniProt)
• [wiki|Sigma-54 factor interaction domain] (aa 368-598) (according to UniProt)

Structure

• [PDB|1OJL] (ZraR from Salmonella typhimurium, C-terminal interaction domain, corresponds to aa 368 ... 683 of BkdR, 41% identity) [pubmed|16005641]
• [AF|P54529]

Biological materials

Mutant

• MGNA-C375 (yqiR::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2373 NBRP B. subtilis, Japan]
• 1A913 ( ''bkdR''::''kan''), [Pubmed|16585774], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A913&Search=1A913 BGSC] and in [wiki|Jörg Stülke]'s lab
• BKE24100 ([gene|4CEBD81F485DD0B660E297FFC34A0F5270652184|bkdR]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE24100 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCCCGATACCCCTTTGTA,  downstream forward: _UP4_TAATTTGCAGAATAAACGCA
• BKK24100 ([gene|4CEBD81F485DD0B660E297FFC34A0F5270652184|bkdR]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK24100 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCCCGATACCCCTTTGTA,  downstream forward: _UP4_TAATTTGCAGAATAAACGCA

References

• 16585774,10094682,23123912,12823818,21906631,16005641