Difference between revisions of "BkdB"

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= [[Categories]] containing this gene/protein =
 
= [[Categories]] containing this gene/protein =
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* '''Protein family:''' 2-oxoacid dehydrogenase family (according to Swiss-Prot)
 
* '''Protein family:''' 2-oxoacid dehydrogenase family (according to Swiss-Prot)
  
* '''Paralogous protein(s):'''
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* '''Paralogous protein(s):''' [[AcoC]], [[OdhB]], [[PdhC]]
  
 
=== Extended information on the protein ===
 
=== Extended information on the protein ===
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* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=bkdB_2496796_2498070_-1 bkdB] {{PubMed|22383849}}
 
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=bkdB_2496796_2498070_-1 bkdB] {{PubMed|22383849}}
  
* '''Sigma factor:''' [[SigL]] [http://www.ncbi.nlm.nih.gov/sites/entrez/10094682 PubMed]
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* '''[[Sigma factor]]:''' [[SigL]] [http://www.ncbi.nlm.nih.gov/sites/entrez/10094682 PubMed]
  
 
* '''Regulation:'''  
 
* '''Regulation:'''  

Revision as of 19:18, 8 June 2013

  • Description: 2-oxoisovalerate dehydrogenase (E2 subunit, lipoamide acyltransferase)

Gene name bkdB
Synonyms bfmBB, bfmB2, bkd
Essential no
Product 2-oxoisovalerate dehydrogenase
(E2 subunit, lipoamide acyltransferase)
Function utilization of branched-chain keto acids
Gene expression levels in SubtiExpress: bkdB
Metabolic function and regulation of this protein in SubtiPathways:
Lipid synthesis, Ile, Leu, Val
MW, pI 45 kDa, 5.301
Gene length, protein length 1272 bp, 424 aa
Immediate neighbours bmrR, bkdAB
Sequences Protein DNA DNA_with_flanks
Genetic context
BkdB context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
BkdB expression.png















Categories containing this gene/protein

utilization of amino acids

This gene is a member of the following regulons

BkdR regulon, CodY regulon, SigL regulon

The gene

Basic information

  • Locus tag: BSU24030

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: 2-methylpropanoyl-CoA + enzyme N(6)-(dihydrolipoyl)lysine = CoA + enzyme N(6)-(S-(2-methylpropanoyl)dihydrolipoyl)lysine (according to Swiss-Prot)
  • Protein family: 2-oxoacid dehydrogenase family (according to Swiss-Prot)

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • induced in the presence of isoleucine or valine (BkdR) PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Jean-Christophe Meile, Ling Juan Wu, S Dusko Ehrlich, Jeff Errington, Philippe Noirot
Systematic localisation of proteins fused to the green fluorescent protein in Bacillus subtilis: identification of new proteins at the DNA replication factory.
Proteomics: 2006, 6(7);2135-46
[PubMed:16479537] [WorldCat.org] [DOI] (P p)

M Nickel, G Homuth, C Böhnisch, U Mäder, T Schweder
Cold induction of the Bacillus subtilis bkd operon is mediated by increased mRNA stability.
Mol Genet Genomics: 2004, 272(1);98-107
[PubMed:15241682] [WorldCat.org] [DOI] (P p)

Ken-ichi Yoshida, Hirotake Yamaguchi, Masaki Kinehara, Yo-hei Ohki, Yoshiko Nakaura, Yasutaro Fujita
Identification of additional TnrA-regulated genes of Bacillus subtilis associated with a TnrA box.
Mol Microbiol: 2003, 49(1);157-65
[PubMed:12823818] [WorldCat.org] [DOI] (P p)

Tanja Kaan, Georg Homuth, Ulrike Mäder, Julia Bandow, Thomas Schweder
Genome-wide transcriptional profiling of the Bacillus subtilis cold-shock response.
Microbiology (Reading): 2002, 148(Pt 11);3441-3455
[PubMed:12427936] [WorldCat.org] [DOI] (P p)

M Debarbouille, R Gardan, M Arnaud, G Rapoport
Role of bkdR, a transcriptional activator of the sigL-dependent isoleucine and valine degradation pathway in Bacillus subtilis.
J Bacteriol: 1999, 181(7);2059-66
[PubMed:10094682] [WorldCat.org] [DOI] (P p)