BioI

• Description: cytochrome P450 enzyme
  
  

Gene name	bioI
Synonyms
Essential	no
Product	cytochrome P450 enzyme
Function	biosynthesis of biotin
Gene expression levels in SubtiExpress: bioI
Interactions involving this protein in SubtInteract: BioI
Metabolic function and regulation of this protein in SubtiPathways: Biotin
MW, pI	44 kDa, 6.2
Gene length, protein length	1185 bp, 395 aa
Immediate neighbours	ytbQ, bioB
Sequences	Protein DNA Advanced_DNA
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed 500px

Categories containing this gene/protein

biosynthesis of cofactors

This gene is a member of the following regulons

BirA regulon

The gene

Basic information

• Locus tag: BSU30190

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: formation of pimelic acid via a multiple-step oxidative cleavage of long-chain fatty acids

• Protein family: cytochrome P450 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • Fer-BioI PubMed
  • YkuP-BioI PubMed
  • AcpA-BioI PubMed

• Localization:

Database entries

• Structure: 3EJB, 3EJE (complex with AcpA) PubMed

• UniProt: P53554

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: bioW-bioA-bioF-bioD-bioB-bioI-ytbQ PubMed

• Expression browser: bioI PubMed

• Sigma factor:

• Regulation:
  • repressed in the presence of biotin (BirA) PubMed

• Regulatory mechanism:
  • BirA: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Original publications

Max J Cryle
Selectivity in a barren landscape: the P450(BioI)-ACP complex.
Biochem Soc Trans: 2010, 38(4);934-9
[PubMed:20658980] [WorldCat.org] [DOI] (I p)

Max J Cryle, Ilme Schlichting
Structural insights from a P450 Carrier Protein complex reveal how specificity is achieved in the P450(BioI) ACP complex.
Proc Natl Acad Sci U S A: 2008, 105(41);15696-701
[PubMed:18838690] [WorldCat.org] [DOI] (I p)

Rachel J Lawson, David Leys, Michael J Sutcliffe, Carol A Kemp, Myles R Cheesman, Susan J Smith, John Clarkson, W Ewen Smith, Ihtshamul Haq, John B Perkins, Andrew W Munro
Thermodynamic and biophysical characterization of cytochrome P450 BioI from Bacillus subtilis.
Biochemistry: 2004, 43(39);12410-26
[PubMed:15449931] [WorldCat.org] [DOI] (P p)

Rachel J Lawson, Claes von Wachenfeldt, Ihtshamul Haq, John Perkins, Andrew W Munro
Expression and characterization of the two flavodoxin proteins of Bacillus subtilis, YkuN and YkuP: biophysical properties and interactions with cytochrome P450 BioI.
Biochemistry: 2004, 43(39);12390-409
[PubMed:15449930] [WorldCat.org] [DOI] (P p)

Max J Cryle, James J De Voss
Carbon-carbon bond cleavage by cytochrome p450(BioI)(CYP107H1).
Chem Commun (Camb): 2004, (1);86-7
[PubMed:14737344] [WorldCat.org] [DOI] (P p)

Max J Cryle, Nick J Matovic, James J De Voss
Products of cytochrome P450(BioI) (CYP107H1)-catalyzed oxidation of fatty acids.
Org Lett: 2003, 5(18);3341-4
[PubMed:12943422] [WorldCat.org] [DOI] (P p)

Amanda J Green, Andrew W Munro, Myles R Cheesman, Graeme A Reid, Claes von Wachenfeldt, Stephen K Chapman
Expression, purification and characterisation of a Bacillus subtilis ferredoxin: a potential electron transfer donor to cytochrome P450 BioI.
J Inorg Biochem: 2003, 93(1-2);92-9
[PubMed:12538057] [WorldCat.org] [DOI] (P p)

A J Green, S L Rivers, M Cheeseman, G A Reid, L G Quaroni, I D Macdonald, S K Chapman, A W Munro
Expression, purification and characterization of cytochrome P450 Biol: a novel P450 involved in biotin synthesis in Bacillus subtilis.
J Biol Inorg Chem: 2001, 6(5-6);523-33
[PubMed:11472016] [WorldCat.org] [DOI] (P p)

J E Stok, J De Voss
Expression, purification, and characterization of BioI: a carbon-carbon bond cleaving cytochrome P450 involved in biotin biosynthesis in Bacillus subtilis.
Arch Biochem Biophys: 2000, 384(2);351-60
[PubMed:11368323] [WorldCat.org] [DOI] (P p)

J B Perkins, S Bower, C L Howitt, R R Yocum, J Pero
Identification and characterization of transcripts from the biotin biosynthetic operon of Bacillus subtilis.
J Bacteriol: 1996, 178(21);6361-5
[PubMed:8892842] [WorldCat.org] [DOI] (P p)

S Bower, J B Perkins, R R Yocum, C L Howitt, P Rahaim, J Pero
Cloning, sequencing, and characterization of the Bacillus subtilis biotin biosynthetic operon.
J Bacteriol: 1996, 178(14);4122-30
[PubMed:8763940] [WorldCat.org] [DOI] (P p)