BglP

• Description: trigger enzyme: beta-glucoside-specific phosphotransferase system, EIIBC
  
  

Gene name	bglP
Synonyms	sytA
Essential	no
Product	trigger enzyme: beta-glucoside-specific phosphotransferase system, EIIBC
Function	beta-glucoside uptake and phosphorylation, control of LicT activity
Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism
MW, pI	64 kDa, 6.809
Gene length, protein length	1827 bp, 609 aa
Immediate neighbours	bglH, yxxE
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU39270

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L-histidine/cysteine (according to Swiss-Prot)

• Protein family: PTS permease, sucrose permease (Scr) family PubMed

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P40739

• KEGG entry: [3]

• E.C. number: 2.7.1.69 9

Expression and regulation

• Operon: bglP-bglH-yxiE PubMed

• Sigma factor: SigA

• Regulation:
  • repressed by glucose (CcpA, LicT)
  • induced by salicin (LicT) PubMed

• Regulatory mechanism:
  • CcpA: transcription repression, (CcpA binding site overlaps -35 region)
  • LicT-dependent RNA switch (antitermination, lack of LicT-dependent antitermination in the presence of gucose due to the requirement of LicT to be phosphorylated by HPr

• Additional information:

Biological materials

• Mutant: GP475 (erm), available in the Stülke lab

• Expression vector:

• lacZ fusion: pGP600 (in pAC6), available in Stülke lab

• GFP fusion:

• two-hybrid system:

• Antibody: