Difference between revisions of "BglP"

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(References)
(Expression and regulation)
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* '''[[Sigma factor]]:''' [[SigA]]
 
* '''[[Sigma factor]]:''' [[SigA]]
  
* '''Regulation:''' repressed by glucose ([[CcpA]]) induced by salicin  [http://www.ncbi.nlm.nih.gov/sites/entrez/7883710 PubMed], repressed by glucose (catabolite repression)
+
* '''Regulation:'''  
 +
** repressed by glucose ([[CcpA]], [[LicT]])  
 +
** induced by salicin  ([[LicT]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/7883710 PubMed]
  
* '''Regulatory mechanism:''' [[CcpA]]: transcription repression, Induction: [[LicT]]-dependent RNA switch (antitermination), catabolite repression: repression by [[CcpA]] ([[CcpA]] binding site overlaps -35 region) and lack of [[LicT]]-dependent antitermination in the presence of gucose due to the requirement of [[LicT]] to be phosphorylated by [[PtsH| HPr]]
+
* '''Regulatory mechanism:'''  
 +
** [[CcpA]]: transcription repression, ([[CcpA]] binding site overlaps -35 region)
 +
** [[LicT]]-dependent [[RNA switch]] (antitermination, lack of [[LicT]]-dependent antitermination in the presence of gucose due to the requirement of [[LicT]] to be phosphorylated by [[PtsH| HPr]]
  
 
* '''Additional information:'''
 
* '''Additional information:'''

Revision as of 20:03, 8 June 2009

  • Description: trigger enzyme: beta-glucoside-specific phosphotransferase system, EIIBC

Gene name bglP
Synonyms sytA
Essential no
Product trigger enzyme: beta-glucoside-specific phosphotransferase system, EIIBC
Function beta-glucoside uptake and phosphorylation, control of LicT activity
MW, pI 64 kDa, 6.809
Gene length, protein length 1827 bp, 609 aa
Immediate neighbours bglH, yxxE
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
BglP context.gif
This image was kindly provided by SubtiList





The gene

Basic information

  • Locus tag: BSU39270

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L-histidine/cysteine (according to Swiss-Prot)
  • Protein family: PTS permease, sucrose permease (Scr) family PubMed
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: cell membrane (according to Swiss-Prot)

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulatory mechanism:
    • CcpA: transcription repression, (CcpA binding site overlaps -35 region)
    • LicT-dependent RNA switch (antitermination, lack of LicT-dependent antitermination in the presence of gucose due to the requirement of LicT to be phosphorylated by HPr
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Jonathan Reizer, Steffi Bachem, Aiala Reizer, Maryvonne Arnaud, Milton H Saier, Jörg Stülke
Novel phosphotransferase system genes revealed by genome analysis - the complete complement of PTS proteins encoded within the genome of Bacillus subtilis.
Microbiology (Reading): 1999, 145 ( Pt 12);3419-3429
[PubMed:10627040] [WorldCat.org] [DOI] (P p)

S Krüger, S Gertz, M Hecker
Transcriptional analysis of bglPH expression in Bacillus subtilis: evidence for two distinct pathways mediating carbon catabolite repression.
J Bacteriol: 1996, 178(9);2637-44
[PubMed:8626332] [WorldCat.org] [DOI] (P p)

S Krüger, M Hecker
Regulation of the putative bglPH operon for aryl-beta-glucoside utilization in Bacillus subtilis.
J Bacteriol: 1995, 177(19);5590-7
[PubMed:7559347] [WorldCat.org] [DOI] (P p)

D Le Coq, C Lindner, S Krüger, M Steinmetz, J Stülke
New beta-glucoside (bgl) genes in Bacillus subtilis: the bglP gene product has both transport and regulatory functions similar to those of BglF, its Escherichia coli homolog.
J Bacteriol: 1995, 177(6);1527-35
[PubMed:7883710] [WorldCat.org] [DOI] (P p)


  1. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed