bglP
168
beta-glucoside permease of the [wiki|phosphotransferase systems|phosphotransferase system], EIIBCA of the [category|SW.1.2.2|PTS], [category|SW.3.4.3|Trigger enzyme], control of [protein|FAC682CCD66056A8CBB9AE5F4BF51C1AADEBBD2C|licT] activity
locus
BSU_39270
Molecular weight
64.52 kDa
pI
6.81
function
beta-glucoside uptake and phosphorylation, control of [protein|FAC682CCD66056A8CBB9AE5F4BF51C1AADEBBD2C|licT] activity
product
beta-glucoside permease of the [wiki|phosphotransferase systems|phosphotransferase system]
essential
no
ec
2.7.1.69
synonyms
bglP, sytA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG2190 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
4,033,778 4,035,607
The protein
Protein family
[category|SW.1.2.2|PTS] permease, sucrose family [Pubmed|10627040]
[wiki|Domains]
[wiki|PTS EIIA domain] type-1 (aa 480-584) (according to UniProt)
[wiki|PTS EIIB domain] type-1 (aa 1-86) (according to UniProt)
[wiki|PTS EIIC domain] type-1 (aa 103-459) (according to UniProt)
Structure
[AF|P40739]
Paralogous protein(s)
[protein|531F132F7F6A878F1E1D56977B9898A14272349A|sacX], [protein|AE02C38397AA790E4B216BB6DBABFE907B984D05|sacP], [protein|BC568649A341B2E6341993EDA4BF52BDE18A3294|treP], [protein|178D5E2AA1225FE909E6F2B63B3595F5ABB8E7A2|murP]
[wiki|Localization]
cell membrane [Pubmed|23475962]
Biological materials
Mutant
GP475 (erm), available in [wiki|Jörg Stülke]'s lab [pubmed|23475962]
BKE39270 ([gene|35E6C81BE481C3E57CF47B781580368C3AC15D83|bglP]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE39270 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTGTATACCTCCTTTTT, downstream forward: _UP4_TGAAAAAACTAATGGGGTGA
BKK39270 ([gene|35E6C81BE481C3E57CF47B781580368C3AC15D83|bglP]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK39270 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTGTATACCTCCTTTTT, downstream forward: _UP4_TGAAAAAACTAATGGGGTGA
Expression vectors
pGP1290 (C-terminal Strep-tag, purification from ''B. subtilis'', for [wiki|SPINE], in [wiki|pGP382]), available in [wiki|Jörg Stülke]'s lab,
pGP1300 (expression of ''[gene|35E6C81BE481C3E57CF47B781580368C3AC15D83|bglP]'' in ''B. subtilis'', in [wiki|pBQ200]), available in [wiki|Jörg Stülke]'s lab
lacZ fusion
pGP600 (in [wiki|pAC6]), available in [wiki|Jörg Stülke]'s lab
GFP fusion
GP1266, [gene|35E6C81BE481C3E57CF47B781580368C3AC15D83|bglP]-cfp, available in [wiki|Jörg Stülke]'s lab [pubmed|23475962]
References
Page visits: 6143
Time of last update: 2024-04-24 11:18:03
Author of last update: Christoph.elfmann