bglP

168

beta-glucoside permease of the [wiki|phosphotransferase systems|phosphotransferase system], EIIBCA of the [category|SW.1.2.2|PTS], [category|SW.3.4.3|Trigger enzyme], control of [protein|FAC682CCD66056A8CBB9AE5F4BF51C1AADEBBD2C|licT] activity

locus: BSU_39270

Molecular weight: 64.52 kDa

pI: 6.81

Protein length: 609 aa

Gene length: 1830 bp

function: beta-glucoside uptake and phosphorylation, control of [protein|FAC682CCD66056A8CBB9AE5F4BF51C1AADEBBD2C|licT] activity

product: beta-glucoside permease of the [wiki|phosphotransferase systems|phosphotransferase system]

essential: no

ec: 2.7.1.69

synonyms: bglP, sytA

List of homologs in different organisms, belongs to COG2190 (Galperin et al., 2021)

Gene

Coordinates: 4,033,778  4,035,607

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The protein

Protein family

• [category|SW.1.2.2|PTS] permease, sucrose family [Pubmed|10627040]

[wiki|Domains]

• [wiki|PTS EIIA domain] type-1 (aa 480-584) (according to UniProt)
• [wiki|PTS EIIB domain] type-1 (aa 1-86) (according to UniProt)
• [wiki|PTS EIIC domain] type-1 (aa 103-459) (according to UniProt)

Structure

• [AF|P40739]

Paralogous protein(s)

• [protein|531F132F7F6A878F1E1D56977B9898A14272349A|sacX], [protein|AE02C38397AA790E4B216BB6DBABFE907B984D05|sacP], [protein|BC568649A341B2E6341993EDA4BF52BDE18A3294|treP], [protein|178D5E2AA1225FE909E6F2B63B3595F5ABB8E7A2|murP]

[wiki|Localization]

• cell membrane [Pubmed|23475962]

Biological materials

Mutant

• GP475 (erm), available in [wiki|Jörg Stülke]'s lab [pubmed|23475962]
• BKE39270 ([gene|35E6C81BE481C3E57CF47B781580368C3AC15D83|bglP]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE39270 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTGTATACCTCCTTTTT,  downstream forward: _UP4_TGAAAAAACTAATGGGGTGA
• BKK39270 ([gene|35E6C81BE481C3E57CF47B781580368C3AC15D83|bglP]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK39270 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTGTATACCTCCTTTTT,  downstream forward: _UP4_TGAAAAAACTAATGGGGTGA

Expression vectors

• pGP1290 (C-terminal Strep-tag, purification from ''B. subtilis'', for [wiki|SPINE], in [wiki|pGP382]), available in [wiki|Jörg Stülke]'s lab,
• pGP1300 (expression of ''[gene|35E6C81BE481C3E57CF47B781580368C3AC15D83|bglP]'' in ''B. subtilis'', in [wiki|pBQ200]), available in [wiki|Jörg Stülke]'s lab

lacZ fusion

• pGP600 (in [wiki|pAC6]), available in [wiki|Jörg Stülke]'s lab

GFP fusion

• GP1266, [gene|35E6C81BE481C3E57CF47B781580368C3AC15D83|bglP]-cfp, available in [wiki|Jörg Stülke]'s lab [pubmed|23475962]

References

• 16672620,20525796,10627040,7883710,8626332,7559347,22900538,23475962