Difference between revisions of "BglP"

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(Biological materials)
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|style="background:#ABCDEF;" align="center"| '''Product''' || [[trigger enzymes|trigger enzyme]]: beta-glucoside-specific <br/>phosphotransferase system, EIIBC  
 
|style="background:#ABCDEF;" align="center"| '''Product''' || [[trigger enzymes|trigger enzyme]]: beta-glucoside-specific <br/>phosphotransferase system, EIIBC  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || beta-glucoside uptake and phosphorylation, <br/>control of LicT activity
+
|style="background:#ABCDEF;" align="center"|'''Function''' || beta-glucoside uptake and phosphorylation, <br/>control of [[LicT]] activity
 
|-
 
|-
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/carbohydrate_metabolic_pathways.html Sugar catabolism]'''
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/carbohydrate_metabolic_pathways.html Sugar catabolism]'''
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* '''Catalyzed reaction/ biological activity:''' Protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L-histidine/cysteine (according to Swiss-Prot)  
 
* '''Catalyzed reaction/ biological activity:''' Protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L-histidine/cysteine (according to Swiss-Prot)  
  
* '''Protein family:''' PTS permease, sucrose permease (Scr) family [http://www.ncbi.nlm.nih.gov/pubmed/10627040 PubMed]  
+
* '''Protein family:''' [[PTS]] permease, sucrose permease (Scr) family [http://www.ncbi.nlm.nih.gov/pubmed/10627040 PubMed]  
  
 
* '''Paralogous protein(s):'''
 
* '''Paralogous protein(s):'''
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* '''Expression vector:'''
 
* '''Expression vector:'''
 
          
 
          
* '''lacZ fusion:'''
+
* '''lacZ fusion:''' pGP600 (in [[pAC6]]), available in [[Stülke]] lab
  
 
* '''GFP fusion:'''
 
* '''GFP fusion:'''

Revision as of 18:36, 29 October 2009

  • Description: trigger enzyme: beta-glucoside-specific phosphotransferase system, EIIBC

Gene name bglP
Synonyms sytA
Essential no
Product trigger enzyme: beta-glucoside-specific
phosphotransferase system, EIIBC
Function beta-glucoside uptake and phosphorylation,
control of LicT activity
Metabolic function and regulation of this protein in SubtiPathways:
Sugar catabolism
MW, pI 64 kDa, 6.809
Gene length, protein length 1827 bp, 609 aa
Immediate neighbours bglH, yxxE
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
BglP context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU39270

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L-histidine/cysteine (according to Swiss-Prot)
  • Protein family: PTS permease, sucrose permease (Scr) family PubMed
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: cell membrane (according to Swiss-Prot)

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulatory mechanism:
    • CcpA: transcription repression, (CcpA binding site overlaps -35 region)
    • LicT-dependent RNA switch (antitermination, lack of LicT-dependent antitermination in the presence of gucose due to the requirement of LicT to be phosphorylated by HPr
  • Additional information:

Biological materials

  • Mutant: GP475 (erm), available in the Stülke lab
  • Expression vector:
  • lacZ fusion: pGP600 (in pAC6), available in Stülke lab
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References