Bcd

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  • Description: valine dehydrogenase, isoleucine dehydrogenase, L-leucine dehydrogenase

Gene name bcd
Synonyms bkd, yqiT
Essential no
Product valine dehydrogenase, isoleucine dehydrogenase,
L-leucine dehydrogenase
Function utilization of branched-chain keto acids
Gene expression levels in SubtiExpress: bcd
Metabolic function and regulation of this protein in SubtiPathways:
bcd
MW, pI 39 kDa, 4.942
Gene length, protein length 1092 bp, 364 aa
Immediate neighbours buk, ptb
Sequences Protein DNA DNA_with_flanks
Genetic context
Bcd context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
Bcd expression.png






























Categories containing this gene/protein

utilization of amino acids

This gene is a member of the following regulons

BkdR regulon, CodY regulon, SigL regulon

The gene

Basic information

  • Locus tag: BSU24080

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: L-leucine + H2O + NAD+ = 4-methyl-2-oxopentanoate + NH3 + NADH (according to Swiss-Prot)
  • Protein family: Glu/Leu/Phe/Val dehydrogenases family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 1LEH (from Lysinibacillus sphaericus, 71% identity, 82% similarity) PubMed
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • induced in the presence of isoleucine or valine (BkdR) PubMed
  • Additional information:
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 565 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 3502 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 2690 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 11556 PubMed

Biological materials

  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

N M Brunhuber, J S Blanchard
The biochemistry and enzymology of amino acid dehydrogenases.
Crit Rev Biochem Mol Biol: 1994, 29(6);415-67
[PubMed:7705101] [WorldCat.org] [DOI] (P p)

Original publications