bacD

168

alanine-anticapsin ligase

locus: BSU_37710

Molecular weight: 52.10 kDa

pI: 4.68

Protein length: 472 aa

Gene length: 1419 bp

function: biosynthesis of the antibiotic bacilysin

product: alanine-anticapsin ligase

essential: no

ec: 6.3.2.28

synonyms: bacD, ywfE, ipa-83d

List of homologs in different organisms, belongs to COG0439 (Galperin et al., 2021)

Gene

Coordinates: 3,870,668  3,872,086

Phenotypes of a mutant

• a ''bacD'' point mutation has been isolated upon selction at low pressure [Pubmed|26296725]

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The protein

Catalyzed reaction/ biological activity

• ligation of anticapsin to L-Ala to form bacilysin, ultimate step in bacilysin biosynthesis [Pubmed|23317005]
• ATP + L-alanine + L-anticapsin --> ADP + bacilysin + H⁺ + phosphate (according to UniProt)

[wiki|Domains]

• [wiki|ATP-grasp doamin] (aa 142-355) (according to UniProt)

Structure

• [PDB|3VMM] [Pubmed|22407814]
• [AF|P39641]

Biological materials

Mutant

• MGNA-B241 (ywfE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1240 NBRP B. subtilis, Japan]
• BKE37710 ([gene|2CE0C0484A70EEE40277996CD3D564A84FEAF5B3|bacD]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE37710 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CTCCATATGTAAAACACTCC,  downstream forward: _UP4_ACGGCAAAGTATGTGCTGCC
• BKK37710 ([gene|2CE0C0484A70EEE40277996CD3D564A84FEAF5B3|bacD]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK37710 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CTCCATATGTAAAACACTCC,  downstream forward: _UP4_ACGGCAAAGTATGTGCTGCC

References

• 16030213,12372825,19352016,19801406,21948839,22407814,24702628,23317005,12697329,21709425,22298000,26296725