BacB

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  • Description: oxidase that catalyzes the synthesis of 2-oxo-3-(4-oxocyclohexa-2,5-dienyl)propanoic acid, a precursor to L-anticapsin

Gene name bacB
Synonyms ywfC, ipa-81d
Essential no
Product oxidase
Function biosynthesis of the antibiotic bacilysin
MW, pI 26 kDa, 5.193
Gene length, protein length 705 bp, 235 aa
Immediate neighbours bacC, bacA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YwfC context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU37730

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: synthesis of 2-oxo-3-(4-oxocyclohexa-2,5-dienyl)propanoic acid, a precursor to L-anticapsin PubMed
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

M Rajavel, B Gopal
Analysis of multiple crystal forms of Bacillus subtilis BacB suggests a role for a metal ion as a nucleant for crystallization.
Acta Crystallogr D Biol Crystallogr: 2010, 66(Pt 5);635-9
[PubMed:20445239] [WorldCat.org] [DOI] (I p)

Sarah A Mahlstedt, Christopher T Walsh
Investigation of anticapsin biosynthesis reveals a four-enzyme pathway to tetrahydrotyrosine in Bacillus subtilis.
Biochemistry: 2010, 49(5);912-23
[PubMed:20052993] [WorldCat.org] [DOI] (I p)

Takashi Inaoka, Guojun Wang, Kozo Ochi
ScoC regulates bacilysin production at the transcription level in Bacillus subtilis.
J Bacteriol: 2009, 191(23);7367-71
[PubMed:19801406] [WorldCat.org] [DOI] (I p)

Malligarjunan Rajavel, Ashima Mitra, Balasubramanian Gopal
Role of Bacillus subtilis BacB in the synthesis of bacilysin.
J Biol Chem: 2009, 284(46);31882-92
[PubMed:19776011] [WorldCat.org] [DOI] (I p)

Gerhard Steinborn, Mohammad-Reza Hajirezaei, Jürgen Hofemeister
bac genes for recombinant bacilysin and anticapsin production in Bacillus host strains.
Arch Microbiol: 2005, 183(2);71-9
[PubMed:15609023] [WorldCat.org] [DOI] (P p)

Ayten Yazgan Karataş, Seçil Cetin, Gülay Ozcengiz
The effects of insertional mutations in comQ, comP, srfA, spo0H, spo0A and abrB genes on bacilysin biosynthesis in Bacillus subtilis.
Biochim Biophys Acta: 2003, 1626(1-3);51-6
[PubMed:12697329] [WorldCat.org] (P p)

Takashi Inaoka, Kosaku Takahashi, Mayumi Ohnishi-Kameyama, Mitsuru Yoshida, Kozo Ochi
Guanine nucleotides guanosine 5'-diphosphate 3'-diphosphate and GTP co-operatively regulate the production of an antibiotic bacilysin in Bacillus subtilis.
J Biol Chem: 2003, 278(4);2169-76
[PubMed:12372825] [WorldCat.org] [DOI] (P p)