bacB

168

oxidase that catalyzes the synthesis of 2-oxo-3-(4-oxocyclohexa-2,5-dienyl)propanoic acid, a precursor to L-anticapsin

locus: BSU_37730

Molecular weight: 26.69 kDa

pI: 5.19

Protein length: 235 aa

Gene length: 708 bp

function: biosynthesis of the antibiotic bacilysin

product: oxidase

essential: no

synonyms: bacB, ywfC, ipa-81d

List of homologs in different organisms, belongs to COG1917 (Galperin et al., 2021)

Gene

Coordinates: 3,872,869  3,873,576

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The protein

Catalyzed reaction/ biological activity

• synthesis of 2-oxo-3-(4-oxocyclohexa-2,5-dienyl)propanoic acid, a precursor to L-anticapsin [Pubmed|19776011]
• 3-[(4R)-4-hydroxycyclohexa-1,5-dien-1-yl]-2-oxopropanoate --> 3-[(1E,4R)-4-hydroxycyclohex-2-en-1-ylidene]pyruvate (according to UniProt)

[wiki|Domains]

• 2 [wiki|cupin 2 domain]s (aa 41 ... 106, aa 151 ... 216)

Structure

• [PDB|3H7J] [Pubmed|19776011]
• [AF|P39639]

Biological materials

Mutant

• MGNA-B240 (ywfC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1239 NBRP B. subtilis, Japan]
• BKE37730 ([gene|0EEE23875DB720D256D53F00D1AD5EF24B538B27|bacB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE37730 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_GAAATAAAGCTCCTGCATAT,  downstream forward: _UP4_AAAATGAAGGCGGATGAATG
• BKK37730 ([gene|0EEE23875DB720D256D53F00D1AD5EF24B538B27|bacB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK37730 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_GAAATAAAGCTCCTGCATAT,  downstream forward: _UP4_AAAATGAAGGCGGATGAATG

References

• 15609023,12372825,21948839,19776011,19801406,20052993,20445239