yxjG

168

putative B12-independent methionine synthase

locus: BSU_38960

Molecular weight: 42.96 kDa

pI: 5.01

Protein length: 378 aa

Gene length: 1137 bp

function: unknown

product: putative methionine synthase

essential: no

synonyms: yxjG

List of homologs in different organisms, belongs to COG0620 (Galperin et al., 2021)

Gene

Coordinates: 3,999,350  4,000,486

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The protein

Structure

• [PDB|1YPX] (the enzyme from ''Listeria monocytogenes'', 49% identity)
• [AF|P42318]

Modification

• S-bacillithiolation upon hypochlorite stress on Cys-346 [Pubmed|21749987]

Paralogous protein(s)

• [protein|55459D8F0BBB2669EB9F25276A17F1A7EB322AF1|yxjH]

Expression and Regulation

Operons

• genes: [gene|A866ACE8431EC3FF98ADC734D9322AE699ECBFAD|yxjG]

  description: [Pubmed|10746760]

  regulation

  • induced by methionine starvation ([wiki|S-box]) [Pubmed|10094622]
  • the [wiki|S-box] RNA is degraded by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [pubmed|29794222]

  regulatory mechanism

  • S-box: RNA switch, , the [wiki|S-box] [wiki|riboswitch] binds S-adenosylmethionine resulting in termination [Pubmed|10094622], in [regulon|other_regulator:S-box|S-box]

Biological materials

Mutant

• MGNA-B731 (yxjG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1730 NBRP B. subtilis, Japan]
• BKE38960 ([gene|A866ACE8431EC3FF98ADC734D9322AE699ECBFAD|yxjG]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE38960 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTTGTCCATTCCTTCTT,  downstream forward: _UP4_TAAGCAAAGAAAAAAACACA
• BKK38960 ([gene|A866ACE8431EC3FF98ADC734D9322AE699ECBFAD|yxjG]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK38960 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTTGTCCATTCCTTCTT,  downstream forward: _UP4_TAAGCAAAGAAAAAAACACA

References

• 19258532,10094622,18039762,12107147,21749987,29794222