efeO

efeO
168

lipoprotein, elemental iron uptake system (binding protein), high affinity uptake of ferric iron (Fe(III))

locus
BSU_38270
Molecular weight
42.63 kDa
pI
4.71
Protein length
Gene length
function
elemental iron uptake
product
elemental iron uptake system (binding protein)
essential
no
synonyms
efeO, ywbM, efeM

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG2822 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,927,951 → 3,929,108
Phenotypes of a mutant
no growth with ferric iron (Fe3+) as the sole source of iron [pubmed|23764491]
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The protein
Catalyzed reaction/ biological activity
binds ferric iron (Fe(III)) with strong selectivity [Pubmed|23764491]
Protein family
EfeM/EfeO family (single member, according to UniProt)
Structure
[PDB|3AT7] (from ''Sphingomonas sp.'', 40% identity) [Pubmed|21238429]
[AF|P39596]
[wiki|Localization]
membrane [Pubmed|18763711]
extracytosolic lipoprotein [Pubmed|23764491]
Expression and Regulation
Operons
genes
[gene|3BED98E311EF380AE77327C7F0A83254805089C0|efeU]-[gene|B31884DC49684A276D65E21A5F59AFDADEB9749C|efeO]-[gene|3985F88334D7B034A66473C5BB39188AB61A65F6|efeB]
description
[Pubmed|9353933]
regulation
immediately induced upon iron starvation (first wave to allow iron uptake) ([protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur]) [Pubmed|29133393,12354229]
regulatory mechanism
[protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur]: repression, [pubmed|12354229], in [regulon|protein:F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|23764491], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
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[gene|3BED98E311EF380AE77327C7F0A83254805089C0|efeU]→[gene|3985F88334D7B034A66473C5BB39188AB61A65F6|efeB]

2024-03-24 18:03:32

Jstuelk

78

2df6f8b75b6dc01eab7622f9ec49df472f6aad6f

0A342568538EE157691477116B943A8E96AC21D0

Biological materials
Mutant
MGNA-B227 (ywbM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1226 NBRP B. subtilis, Japan]
BKE38270 (Δ[gene|B31884DC49684A276D65E21A5F59AFDADEB9749C|efeO]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE38270 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TGATACAGCGATTTTTGTGA,  downstream forward: _UP4_CTATAAAAGGAGTTTTGTAA
BKK38270 (Δ[gene|B31884DC49684A276D65E21A5F59AFDADEB9749C|efeO]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK38270 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TGATACAGCGATTTTTGTGA,  downstream forward: _UP4_CTATAAAAGGAGTTTTGTAA
References
16672620,9353933,12354229,18763711,23180473,23764491,21238429,27795321,29133393

B31884DC49684A276D65E21A5F59AFDADEB9749C

Page visits: 3056

Time of last update: 2024-03-28 01:06:21

Author of last update: Robert.warneke