narI

narI
168

nitrate reductase (gamma subunit)

locus
BSU_37250
Molecular weight
25.15 kDa
pI
10.04
Protein length
Gene length
function
nitrate respiration, nitrogen assimilation
product
nitrate reductase (gamma subunit)
essential
no
synonyms
narI

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG2181 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,823,558  3,824,229
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The protein
Catalyzed reaction/ biological activity
quinol + nitrate --> quinone + H2O + nitrite (according to UniProt)
[wiki|Cofactors]
heme b [Pubmed|11289299]
Structure
[PDB|1Q16] (the [protein|B992698A12EE9D17DCEF075F82396D5456D35A20|narG]-[protein|6181C785427277C097B81D67A93213AEAF701FF4|narH]-[protein|20060F4C1939FB9A10CB279745D75D0A16627EC6|narI] complex of E. coli, 37% identity) [pubmed|12910261]
[AF|P42177]
[wiki|Localization]
cell membrane (according to UniProt)
Expression and Regulation
Operons
genes
[gene|B992698A12EE9D17DCEF075F82396D5456D35A20|narG]-[gene|6181C785427277C097B81D67A93213AEAF701FF4|narH]-[gene|5528AD62C1BF608D181C8960C88620B63B1E60A0|narJ]-[gene|20060F4C1939FB9A10CB279745D75D0A16627EC6|narI]
description
[Pubmed|8846791,16428414]
regulation
expressed under anaerobic conditions ([protein|search|Fnr]) [Pubmed|8846791,16428414]
regulatory mechanism
[protein|7165CC59CDAF64AAE2D591936860304A53BE5DF6|fnr]: activation, [Pubmed|8846791,16428414], in [regulon|protein:7165CC59CDAF64AAE2D591936860304A53BE5DF6|fnr regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|8846791,16428414], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
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[gene|B992698A12EE9D17DCEF075F82396D5456D35A20|narG]→[gene|20060F4C1939FB9A10CB279745D75D0A16627EC6|narI]

2024-03-24 10:37:27

ghost

55

2df0c9c15bd640f1035afac39ab71391c14fde4b

655A38484A21CE64690D3913F055D0C46476DC9F

Biological materials
Mutant
BKE37250 ([gene|20060F4C1939FB9A10CB279745D75D0A16627EC6|narI]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE37250 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_AATACCCCAGAGGATCTGCC,  downstream forward: _UP4_TGAAAATCCCCCTTGCAAGA
BKK37250 ([gene|20060F4C1939FB9A10CB279745D75D0A16627EC6|narI]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK37250 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_AATACCCCAGAGGATCTGCC,  downstream forward: _UP4_TGAAAATCCCCCTTGCAAGA
References
Reviews
11289299,33705278
Original Publications
9352926,8846791,16428414,12910261

20060F4C1939FB9A10CB279745D75D0A16627EC6

Page visits: 1791

Time of last update: 2024-03-29 10:57:31

Author of last update: Jstuelk