moaA

168

molybdopterin precursor biosynthesis

locus: BSU_36700

Molecular weight: 38.39 kDa

pI: 9.28

Protein length: 341 aa

Gene length: 1026 bp

function: nitrate respiration

product: unknown

essential: no

synonyms: moaA, narA, narAB

List of homologs in different organisms, belongs to COG2896 (Galperin et al., 2021)

Gene

Coordinates: 3,772,325  3,773,350

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The protein

Catalyzed reaction/ biological activity

• involved in the first step of molybdenum cofactor biosynthesis (together with [protein|4A8D74B83E20FA138ED66F0D8FD456B69B740D92|ydiG])
• AH₂ + GTP + S-adenosyl-L-methionine --> (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate + 5'-deoxyadenosine + A + H⁺ + L-methionine (according to UniProt)

Protein family

• [wiki|Radical SAM superfamily] (according to UniProt)

[wiki|Cofactors]

• Fe-S cluster [pubmed|29292548]
• S-adenosyl methionine (according to UniProt)

Structure

• [PDB|1TV7] (from Staphylococcus aureus, 49% identity) [pubmed|15317939]
• [AF|P39757]

Expression and Regulation

Operons

• genes: [gene|095DAEED5F22A3BC46614C16CC907A2DB9E32C6E|fdhD]-[gene|19EB8E7CBC0E8D7A6429ADFFF93D1F71EFE3FF11|moaA]

  description: [Pubmed|22383849]

Biological materials

Mutant

• BKE36700 ([gene|19EB8E7CBC0E8D7A6429ADFFF93D1F71EFE3FF11|moaA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE36700 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TGTAATCATAGCTACAGCCC,  downstream forward: _UP4_TAATTTGAAGTCAAAAGCTT
• BKK36700 ([gene|19EB8E7CBC0E8D7A6429ADFFF93D1F71EFE3FF11|moaA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK36700 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TGTAATCATAGCTACAGCCC,  downstream forward: _UP4_TAATTTGAAGTCAAAAGCTT

References

Reviews

• 23539623,25268953

Original Publications

• 7860592,9352926,15317939