flgL

168

flagellar hook-filament junction proteins, thought to form the hook-filament junction upon which the FliD cap is assembled

locus: BSU_35400

Molecular weight: 32.61 kDa

pI: 4.42

Protein length: 298 aa

Gene length: 897 bp

function: motility and chemotaxis

product: flagellar hook-filament junction proteins

essential: no

synonyms: flgL, yviD

List of homologs in different organisms, belongs to COG1344 (Galperin et al., 2021)

Gene

Coordinates: 3,637,338  3,638,234

Phenotypes of a mutant

• the mutation suppresses the mucoid phenotype of ''[gene|86C729C7D5ABEE519ADC4A893940600BBB655EF1|motA]'' or ''[gene|FE56753D061344B0F10A6523F49C5C7356AA40B6|motB]'' mutants [Pubmed|24296669]
• defective in [category|SW.4.1.4|Swarming] motility [pubmed|35638827]

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The protein

Protein family

• bacterial flagellin family (with [protein|5A94391906ABFE70C5BA14E1E75343BC5805D0D9|yvzB] and [protein|E0BB77C8F1220E931D16A1FA6B75AE8907C76FDB|hag], according to UniProt)

Structure

• [PDB|3PWX] (from ''Viubrio parahaemolyticus'', 26% identity)
• [AF|P96501]

[wiki|Localization]

• secreted (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|45F27C0789199626BA90D2C69E8F13525C911478|comFA]-[gene|D290247A43280532947B707F3AF388D7A7F404D3|comFB]-[gene|04D9D70C0BCFDCBD00B5156908C348135080A9C2|comFC]-[gene|049B1EBF64F0EBC04CE6D529C8EDB0B6B2184DF5|yvyF]-[gene|F03144BF8A187C8931938A21433431B8961E8EE7|flgM]-[gene|6F4CD36D4C1EE99EF7A503F44F53AEB8EFFEAB7F|flgN]-[gene|767ADBEF1B40CB3C74116CEA8CFB2FAF19C1FE7B|flgK]-[gene|82AB04023BCB57967C7501E6AEAC4BB593AA6480|flgL]-[gene|E5A59D2AF53FA220BA98D9653589A8F0D24F75B8|yviE]-[gene|6A6CBD75DCC33C804F853F47D37773930D398CFE|fliW]-[gene|93F328524989597C7D2329B25E665496C9631E87|csrA]

  description: [Pubmed|22383849]

  expression

  • expression is heterogeneous [pubmed|35171018]

  regulatory mechanism

  • [protein|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|comK]: activation, [Pubmed|8412657], in [regulon|protein:08CFA2C72931A75532D4289BC1D18A826DE9F9CA|comK regulon]
  • [protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|degU]: activation, [protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|degU]-P, [Pubmed|21736639], in [regulon|protein:D343D2096664A972026D911E7A17A35C7B1CD1C9|degU regulon]
  • [protein|F7FD94CE7AB290FBFD3A9B88F204961668F9B42C|scoC]: repression, [Pubmed|19898538], in [regulon|protein:F7FD94CE7AB290FBFD3A9B88F204961668F9B42C|scoC regulon]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|8412657], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
  • [protein|7024E4162A6D827069F882FDEACA696EBC05DD40|sigD]: sigma factor, [Pubmed|8045879], in [regulon|protein:7024E4162A6D827069F882FDEACA696EBC05DD40|sigD regulon]

• genes: [gene|F03144BF8A187C8931938A21433431B8961E8EE7|flgM]-[gene|6F4CD36D4C1EE99EF7A503F44F53AEB8EFFEAB7F|flgN]-[gene|767ADBEF1B40CB3C74116CEA8CFB2FAF19C1FE7B|flgK]-[gene|82AB04023BCB57967C7501E6AEAC4BB593AA6480|flgL]

  description: [Pubmed|21736639]

• genes: [gene|049B1EBF64F0EBC04CE6D529C8EDB0B6B2184DF5|yvyF]-[gene|F03144BF8A187C8931938A21433431B8961E8EE7|flgM]-[gene|6F4CD36D4C1EE99EF7A503F44F53AEB8EFFEAB7F|flgN]-[gene|767ADBEF1B40CB3C74116CEA8CFB2FAF19C1FE7B|flgK]-[gene|82AB04023BCB57967C7501E6AEAC4BB593AA6480|flgL]

  description: [Pubmed|8045879]

Biological materials

Mutant

• BKE35400 ([gene|82AB04023BCB57967C7501E6AEAC4BB593AA6480|flgL]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE35400 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TTGTGTTACTCTCATGTGAC,  downstream forward: _UP4_TAAGCGGCTCTTAGGAGTTC
• BKK35400 ([gene|82AB04023BCB57967C7501E6AEAC4BB593AA6480|flgL]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK35400 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TTGTGTTACTCTCATGTGAC,  downstream forward: _UP4_TAAGCGGCTCTTAGGAGTTC

References

• 8412657,8045879,24296669,21736639,35638827