fhuC

168

hydroxamate siderophore [wiki|ABC transporter ](ATP-binding protein) (ferrichrome und ferrioxamine)

locus: BSU_33290

Molecular weight: 29.80 kDa

pI: 6.5

Protein length: 269 aa

Gene length: 810 bp

function: siderophore uptake

product: hydroxamate siderophore [wiki|ABC transporter ](ATP-binding protein) (ferrichrome und ferrioxamine)

essential: no

ec: 3.6.3.34

synonyms: fhuC

List of homologs in different organisms, belongs to COG1120 (Galperin et al., 2021)

Gene

Coordinates: 3,415,387 → 3,416,196

Advertisement

The protein

Catalyzed reaction/ biological activity

• ATP + H₂O + Fe³⁺-hydroxamate complex-[hydroxamate-binding protein](Side 1) --> ADP + phosphate + Fe³⁺-hydroxamate complex(Side 2) + [hydroxamate-binding protein] (according to UniProt)

Protein family

• [wiki|ABC transporter superfamily] (according to UniProt)

[wiki|Domains]

• [wiki|ABC transporter domain] (aa 4-240) (according to UniProt)

Structure

• [PDB|4R9U], the ''E. coli'' BtuC-BtuD complex, BtuD shares 34% identity, 55% similarity with FhuC, [Pubmed|25402482]
• [AF|P49938]

Paralogous protein(s)

• [protein|E76640F895261DA34AD2342B54B43D11857AEA9A|fecF], [protein|5C52DB31F378E49AABE5D937DD901A68F3810477|yusV], [protein|473628F86C18FE9957841F3C8B45242C90CB341D|fpbP]

[wiki|Localization]

• cell membrane (according to UniProt)

Biological materials

Mutant

• BKE33290 (Δ[gene|E70BE74D5AB4B06CDFF902E58A0B9EBF477E21EC|fhuC]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE33290 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATTCGGCCCCCTCCTCT,  downstream forward: _UP4_TGAAAAAATCCCGGTTTGAA
• BKK33290 (Δ[gene|E70BE74D5AB4B06CDFF902E58A0B9EBF477E21EC|fhuC]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK33290 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATTCGGCCCCCTCCTCT,  downstream forward: _UP4_TGAAAAAATCCCGGTTTGAA

References

• 10092453,16672620,25402482,12354229,29133393