yusI

168

similar to arsenate reductase, binds to DNA lesions, may be involved in DNA repair

locus: BSU_32810

Molecular weight: 13.77 kDa

pI: 5.59

Protein length: 118 aa

Gene length: 357 bp

function: unknown

product: unknown

essential: no

synonyms: yusI

List of homologs in different organisms, belongs to COG1393 (Galperin et al., 2021)

Gene

Coordinates: 3,366,573  3,366,929

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The protein

Catalyzed reaction/ biological activity

• binds to RNA base DNA lesions [pubmed|37250769]

Protein family

• ArsC family (with [protein|6A38DAA96F7BBF31FD8A4018A8CA7A72F3C28F79|mgsR] and [protein|2C6386E9A63F410558D168798D077DF91590F454|spx], according to UniProt)

Structure

• [PDB|2M46] (from Staphylococcus aureus, 51% identity)
• [AF|O32175]

Expression and Regulation

Operons

• genes: [gene|5278DD377B110CC14E239B9428F1985B472EEDF5|yusI]-[gene|58CF62EBA28E77668A0B5319142C8AC20D81CD8A|gcvH]-[gene|0253D9B030630340551DC2D4F40B3B118DB0EC56|yusG]

  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-B593 (yusI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1592 NBRP B. subtilis, Japan]
• BKE32810 ([gene|5278DD377B110CC14E239B9428F1985B472EEDF5|yusI]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE32810 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCTTCTTCCCCCTCAATA,  downstream forward: _UP4_TAATGTAAATTTTTTTTGAA
• BKK32810 ([gene|5278DD377B110CC14E239B9428F1985B472EEDF5|yusI]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK32810 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCTTCTTCCCCCTCAATA,  downstream forward: _UP4_TAATGTAAATTTTTTTTGAA

Original Publications

• 37250769