gcvH

168

glycine cleavage system protein H, lipoyl-binding site, octanoyltransferase for lipoic acid biosynthesis, required for lipoate transfer to 2-oxo acid dehydrogenases

locus: BSU_32800

Molecular weight: 14.11 kDa

pI: 3.89

Protein length: 127 aa

Gene length: 384 bp

function: glycine utilization

product: glycine cleavage system protein H

essential: no

synonyms: gcvH, yusH

List of homologs in different organisms, belongs to COG0509 (Galperin et al., 2021)

Gene

Coordinates: 3,366,123  3,366,506

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The protein

Catalyzed reaction/ biological activity

• degradation of glycine. [protein|58CF62EBA28E77668A0B5319142C8AC20D81CD8A|gcvH] shuttles the methylamine group of glycine from [protein|20175DF9FCF07C34A05C572B089C33D1E1FE6DF2|gcvPB] to [protein|F6F449170276055E6D60D89E9E17A152A82CA166|gcvT] (according to UniProt)
• assembly of lipoate, and transfer of the cofactor to [protein|2F40086E35FA32136B9A89C530A86D714FE9460C|pdhC], [protein|02BA02D10DFB06E51101D8CF76BCF5BED94D7CA2|odhB], [protein|C4B6C3EE560C8BD353FEABB1A607C89C20DC8D34|acoC], and [protein|262C9FD20C7A70B6F1FEB57735FA800F38EAB25A|bkdB]

Protein family

• gcvH family (single member, according to UniProt)

[wiki|Domains]

• [wiki|Lipoyl-binding domain] (aa 22-104) (according to UniProt)

[wiki|Cofactors]

• lipoic acid (on Lys-63), lipoylated by [protein|19CEB80CF637F1FED7C493EF743B4BFA632C8D44|lipM] [Pubmed|20882995], can probably be removed by [protein|A0A6CB19191A251BB5600C18E039978A9A34933C|srtN] [pubmed|28900027]

Structure

• [PDB|3IFT] (from ''Mycobacterium tuberculosis'', 50% identity) [Pubmed|20364333]
• [AF|O32174]

Expression and Regulation

Operons

• genes: [gene|5278DD377B110CC14E239B9428F1985B472EEDF5|yusI]-[gene|58CF62EBA28E77668A0B5319142C8AC20D81CD8A|gcvH]-[gene|0253D9B030630340551DC2D4F40B3B118DB0EC56|yusG]

  description: [Pubmed|22383849]

• genes: [gene|58CF62EBA28E77668A0B5319142C8AC20D81CD8A|gcvH]-[gene|0253D9B030630340551DC2D4F40B3B118DB0EC56|yusG]

  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-B592 (yusH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1591 NBRP B. subtilis, Japan]
• BKE32800 ([gene|58CF62EBA28E77668A0B5319142C8AC20D81CD8A|gcvH]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE32800 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAATTGAATCCCTCCATTTT,  downstream forward: _UP4_TAAAACAGGATAGCCGTAAA
• BKK32800 ([gene|58CF62EBA28E77668A0B5319142C8AC20D81CD8A|gcvH]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK32800 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAATTGAATCCCTCCATTTT,  downstream forward: _UP4_TAAAACAGGATAGCCGTAAA
• GP2585 ([gene|58CF62EBA28E77668A0B5319142C8AC20D81CD8A|gcvH]::tet comIQ12L) (in DK1042) available in [wiki|Jörg Stülke]'s lab

References

Reviews

• 27074917

Original Publications

• 20882995,21338421,20364333,28900027,29339506,31066113