tlpA

168

membrane-bound chemotaxis receptor, methyl-accepting chemotaxis protein, acts also as pH sensor, responds to decreasing pH as an attractant signal

locus: BSU_31250

Molecular weight: 72.09 kDa

pI: 4.91

Protein length: 662 aa

Gene length: 1989 bp

function: control of chemotaxis, sensing of acidic environments

product: methyl-accepting chemotaxis protein

essential: no

synonyms: tlpA

List of homologs in different organisms, belongs to COG0840 (Galperin et al., 2021)

Gene

Coordinates: 3,208,280  3,210,268

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The protein

[wiki|Domains]

• [wiki|Cache domain] (aa 152-228) (according to UniProt)
• [wiki|HAMP domain] (aa 303-355) (according to UniProt)
• [wiki|Methyl-accepting transducer domain] (aa 374-610) (according to UniProt)

Structure

• [PDB|6S1K] (from E. coli, corresponds to aa 285 ... 552, 33% identity) [pubmed|31925330]
• [AF|P39216]

Paralogous protein(s)

• [protein|4CD44AD8FE68516C84889EAA2137828E06B8A30B|tlpB], [protein|8333CD46F704F03A22482CAA98DEFCC945362A10|mcpC], [protein|E4E1020B799A1B403BDB4847B91B02D64D161AF3|mcpB], [protein|F02E3080214A327E131ECDE0DBC52C57EFDFE2CE|mcpA]

[wiki|Localization]

• cell membrane [Pubmed|21515776],  Polar (Septum) [Pubmed|16479537]
• accumulates at strongly curved membrane areas generated during cell division [Pubmed|26522943]

Expression and Regulation

Operons

• genes: [gene|1DE26CDEE952142C1303C822F0E2A63AE09F721B|tlpA]-[gene|F02E3080214A327E131ECDE0DBC52C57EFDFE2CE|mcpA]

  description: [Pubmed|8188684]

  regulatory mechanism

  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|abrB]: repression, [Pubmed|20817675], in [regulon|protein:E5110D9C36E8C55AFD1419987B14A53232165F20|abrB regulon]

  sigma factors

  • [protein|7024E4162A6D827069F882FDEACA696EBC05DD40|sigD]: sigma factor, [Pubmed|8188684], in [regulon|protein:7024E4162A6D827069F882FDEACA696EBC05DD40|sigD regulon]

  additional information

  • in minimal medium, McpA is present with 15,900 +/- 3,000 molecules per cell [PubMed|21515776]

Biological materials

Mutant

• BKE31250 ([gene|1DE26CDEE952142C1303C822F0E2A63AE09F721B|tlpA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE31250 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTCCTTTTGAACCTTCT,  downstream forward: _UP4_TAATAGGTCTAAAATACTAT
• BKK31250 ([gene|1DE26CDEE952142C1303C822F0E2A63AE09F721B|tlpA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK31250 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTCCTTTTGAACCTTCT,  downstream forward: _UP4_TAATAGGTCTAAAATACTAT

References

Reviews

• 31792011

Original Publications

• 8188684,21515776,16479537,20817675,26522943,31685537,31925330