serB

168

phosphoserine phosphatase, catalyzes the last step in serine biosynthesis

locus: BSU_28940

Molecular weight: 29.36 kDa

pI: 4.67

Protein length: 260 aa

Gene length: 783 bp

function: biosynthesis of serine

product: phosphoserine phoshatase

essential: no

synonyms: serB, ysaA

List of homologs in different organisms, belongs to COG1011 (Galperin et al., 2021)

Gene

Coordinates: 2,958,434  2,959,216

Phenotypes of a mutant

• auxotrophic for serine [Pubmed|28189581]

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The protein

Catalyzed reaction/ biological activity

• phosphoserine - serine + phosphate [Pubmed|28189581]

Protein family

• [wiki|HAD superfamily] (according to UniProt)

Structure

• [PDB|2GFH] (from mouse, 32% identity)
• [AF|P94512]

Paralogous protein(s)

• [protein|2E1E22349750C3EE9B6575EED30A82ED0D5F07F0|yfnB]

Expression and Regulation

Operons

• genes: [gene|50417B2FA0ED2258A13B1E708514F055297599EF|serB]

  description: [pubmed|22383849,8969504]

Biological materials

Mutant

• MGNA-A998 [gene|50417B2FA0ED2258A13B1E708514F055297599EF|serB]::erm, available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/998 NBRP B. subtilis, Japan]
• BKE28940 ([gene|50417B2FA0ED2258A13B1E708514F055297599EF|serB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE28940 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGATCTTCTCCTCTGCTT,  downstream forward: _UP4_TAAAAAAAGCATGATCTCTT
• BKK28940 ([gene|50417B2FA0ED2258A13B1E708514F055297599EF|serB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK28940 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGATCTTCTCCTCTGCTT,  downstream forward: _UP4_TAAAAAAAGCATGATCTCTT

References

• 8969504,27784292,28189581