ysnD

168

inner spore coat protein

locus: BSU_28320

Molecular weight: 11.58 kDa

pI: 8.48

Protein length: 111 aa

Gene length: 336 bp

function: protection of the spore

product: spore coat protein

essential: no

ec: null

synonyms: ysnD

List of homologs in different organisms

Gene

Coordinates: 2,897,788  2,898,123

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The protein

Structure

• [AF|P94563]

[wiki|Localization]

• inner spore coat, localization depends on [protein|CEBEC9CECCF445C40D793E61A2CD23175631A473|safA]  [Pubmed|22171814,19933362]

Expression and Regulation

Operons

• genes: [gene|5FA26477DF2CE415AB20319853FCB77ABAC71316|ysnD]

  description: [Pubmed|12480901]

  regulation

  • expressed during sporulation in the mother cell ([protein|search|SigE], [protein|search|SigK], [protein|search|GerE]) [Pubmed|15699190,15383836,12480901]

  regulatory mechanism

  • [protein|19228BAD44E6DA3DE908DC390FDF628C18D94E65|gerE]: repression, [Pubmed|15383836], in [regulon|protein:19228BAD44E6DA3DE908DC390FDF628C18D94E65|gerE regulon]
  • [protein|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|tnrA]: activation, [Pubmed|25755103], in [regulon|protein:857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|tnrA regulon]

  sigma factors

  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]: sigma factor, [Pubmed|12480901], in [regulon|protein:3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE regulon]
  • [protein|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|sigK]: sigma factor, [Pubmed|15699190,15383836], in [regulon|protein:24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|sigK regulon]

Biological materials

Mutant

• MGNA-A993 (ysnD::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/993 NBRP B. subtilis, Japan]
• BKE28320 ([gene|5FA26477DF2CE415AB20319853FCB77ABAC71316|ysnD]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE28320 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCGTATCACCTTTTCTTT,  downstream forward: _UP4_TAAAGAATACCACAAGGGCC
• BKK28320 ([gene|5FA26477DF2CE415AB20319853FCB77ABAC71316|ysnD]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK28320 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCGTATCACCTTTTCTTT,  downstream forward: _UP4_TAAAGAATACCACAAGGGCC

References

• 19933362,15699190,15383836,12480901,22171814,25755103