mtnN

mtnN
168

methylthioadenosine nucleosidase

locus
BSU_27270
Molecular weight
25.12 kDa
pI
4.84
Protein length
Gene length
function
methionine salvage
product
methylthioadenosine nucleosidase
essential
no
ec
3.2.2.9
synonyms
mtnN, yrrU, mtn, mthA

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0775 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,787,130  2,787,825
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The protein
Catalyzed reaction/ biological activity
H2O + S-adenosyl-L-homocysteine --> adenine + S-(5-deoxy-D-ribos-5-yl)-L-homocysteine (according to UniProt)
H2O + S-methyl-5'-thioadenosine --> adenine + S-methyl-5-thio-D-ribose (according to UniProt)
Protein family
PNP/UDP phosphorylase family (with [protein|3075327DBE55C4F628171AEE248CAED5870693FA|deoD], according to UniProt)
Structure
[PDB|1JYS] (from ''Escherichia coli'', 51% identity, 70% similarity) [Pubmed|11591349]
[AF|O32028]
Expression and Regulation
Operons
genes
[gene|992A24CF9492965BF0E9EEFBB6F0A06871275068|yrrT]-[gene|5B21D1E73A65B13CE4DDA32ABC71EFA54E8D1D8F|mtnN]-[gene|F195C47849F4F6B4C56E3A386F5D8C84326B01C4|mccA]-[gene|314CB4923D15140E72B0ABAFE3E8BE9CA7310E10|mccB]-[gene|177EFFDCA6E3C3BB840EED13D9F2D71F99B833B6|yrhC]
description
[Pubmed|17056751]
regulation
repressed in the presence of cysteine ([protein|search|CymR]) [Pubmed|16513748]
regulatory mechanism
[protein|50930C56C27D22715620A350220E3C56ADB41020|cymR]: repression, [Pubmed|16513748], in [regulon|protein:50930C56C27D22715620A350220E3C56ADB41020|cymR regulon]
[protein|2C6386E9A63F410558D168798D077DF91590F454|spx]: activation, [Pubmed|12642660,16885442], in [regulon|protein:2C6386E9A63F410558D168798D077DF91590F454|spx regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|16513748], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
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[gene|992A24CF9492965BF0E9EEFBB6F0A06871275068|yrrT]→[gene|177EFFDCA6E3C3BB840EED13D9F2D71F99B833B6|yrhC]

2024-03-10 08:58:29

ghost

70

520f018506caa674deba5a8750946c81db19309b

6F8866D3B9D23886BC36B8964568BAC6D245EA17

Biological materials
Mutant
MGNA-A850 (yrrU::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/850 NBRP B. subtilis, Japan]
BKE27270 ([gene|5B21D1E73A65B13CE4DDA32ABC71EFA54E8D1D8F|mtnN]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE27270 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCTATTCACATTCCCTTC,  downstream forward: _UP4_TAAAACGGGGAGAACGTTCT
BKK27270 ([gene|5B21D1E73A65B13CE4DDA32ABC71EFA54E8D1D8F|mtnN]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK27270 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCTATTCACATTCCCTTC,  downstream forward: _UP4_TAAAACGGGGAGAACGTTCT
References
17056751,16513748,12642660,15102328,16885442,24509311

5B21D1E73A65B13CE4DDA32ABC71EFA54E8D1D8F

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Time of last update: 2024-03-28 19:43:49

Author of last update: Melvin.boenninger