dxs

168

1-deoxyxylulose-5-phosphate synthase, first step in the MEP pathway of isoprenoid biosynthesis

locus: BSU_24270

Molecular weight: 69.38 kDa

pI: 6

Protein length: 633 aa

Gene length: 1902 bp

function: biosynthesis of thiamine, MEP pathway of isoprenoid biosynthesis

product: 1-deoxyxylulose-5-phosphate synthase

essential: yes

ec: 2.2.1.7

synonyms: dxs, yqiE

List of homologs in different organisms, belongs to COG1154 (Galperin et al., 2021)

Gene

Coordinates: 2,523,713 → 2,525,614

Phenotypes of a mutant

• essential [Pubmed|12682299]

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The protein

Catalyzed reaction/ biological activity

• D-glyceraldehyde 3-phosphate + H⁺ + pyruvate --> 1-deoxy-D-xylulose 5-phosphate + CO₂ (according to UniProt)

Protein family

• transketolase family (with [protein|000E237B00539E6850B6A50EE01BA02844B6ACBC|tkt], according to UniProt)

[wiki|Cofactors]

• TPP [pubmed|35988322]

Structure

• [PDB|2O1S] (from ''E. coli'', 44% identity, 62% similarity) [Pubmed|17135236]
• [AF|P54523]

Expression and Regulation

Operons

• genes: [gene|EA9FCF84AE4FA80993566B62A9200D4B32AF5670|dxs]-[gene|923C4177500E9678A0B4855F8F481D4A36ECD863|yqxC]-[gene|62F8E0D6BC9DA6390256FA8FB684EA5C9C722AE4|ahrC]-[gene|5680A06A74D3EC6310EF8677795511C75DB3059E|recN]

  description: [pubmed|22383849]

  regulation

  • [protein|CEE73284EFC0DBE8870CE0B474922DED79475A57|rel] dependent downregulation (Class I) during stringent response [Pubmed|11948165]

  regulatory mechanism

  • stringent response: negative regulation, [pubmed|11948165], in [regulon|other_regulator:stringent response|stringent response]

References

Reviews

• 19348578,35988322

Original Publications

• 12882400,17135236,21296950,23840410,27856234,33242110,35643569