coaA

168

type I pantothenate kinase, major enzyme

locus: BSU_23760

Molecular weight: 36.49 kDa

pI: 5.89

Protein length: 319 aa

Gene length: 960 bp

function: biosynthesis of coenzyme A

product: pantothenate kinase, pantetheine kinase

essential: no

ec: 2.7.1.33

synonyms: coaA, yqjS, panK

List of homologs in different organisms, belongs to COG1072 (Galperin et al., 2021)

Gene

Coordinates: 2,468,549 → 2,469,508

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The protein

Catalyzed reaction/ biological activity

• (R)-pantothenate + ATP --> (R)-4'-phosphopantothenate + ADP + H⁺ [https://www.biorxiv.org/content/10.1101/2024.04.17.589903v1.full.pdf bioRxiv]
• pantetheine + ATP --> phosphopantetheine+ ADP + H⁺ [https://www.biorxiv.org/content/10.1101/2024.04.17.589903v1.full.pdf bioRxiv]

Protein family

• prokaryotic pantothenate kinase family (single member, according to UniProt)

Structure

• [PDB|1SQ5] (from ''Escherichia coli'', 49% identity, 67% similarity) [Pubmed|15136582]
• [AF|P54556]

Expression and Regulation

Operons

• genes: [gene|35B40D812B123766B469B8E5C0FE0EB69F671E4E|yqjP]-[gene|0927C7D45721F47F5ED2646CAAC324A308FD7724|yqjQ]-[gene|4E7390A74A8261E8B732E94859E90FE256AD64EE|dsdA]-[gene|B3362449EF3C9D533090FBD7E7333A3CC5D902CB|coaA]-[gene|441DCE4251C83FCBB01BC58720900365BFE7BB3D|yqjT]

  description: [pubmed|22383849]

Biological materials

Mutant

• MGNA-C399 (yqjS::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2397 NBRP B. subtilis, Japan]
• BKE23760 (Δ[gene|B3362449EF3C9D533090FBD7E7333A3CC5D902CB|coaA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE23760 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACTGTTTACACCTGACTCT,  downstream forward: _UP4_GTGTTGGTAAGGAGGGTATG
• BKK23760 (Δ[gene|B3362449EF3C9D533090FBD7E7333A3CC5D902CB|coaA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK23760 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACTGTTTACACCTGACTCT,  downstream forward: _UP4_GTGTTGGTAAGGAGGGTATG
• GP3355 (''[gene|B3362449EF3C9D533090FBD7E7333A3CC5D902CB|coaA]''::lox72), available in [wiki|Jörg Stülke]'s lab
• GP3380 ([gene|B3362449EF3C9D533090FBD7E7333A3CC5D902CB|coaA]::neo trpC2), available in [wiki|Jörg Stülke]'s lab

References

• 1328157,24784177,21551303