scpA

168

part of the [wiki|condensin] complex, chromosomal origin condensation and segregation

locus: BSU_23220

Molecular weight: 29.46 kDa

pI: 4.79

Protein length: 251 aa

Gene length: 756 bp

function: segregation of replication origins

product: DNA segregation and condensation protein

essential: yes

synonyms: scpA, ypuG

List of homologs in different organisms, belongs to COG1354 (Galperin et al., 2021)

Gene

Coordinates: 2,425,831  2,426,586

Phenotypes of a mutant

• essential [Pubmed|12682299]
• ''[gene|8DD968C97FDC0E81293887E2FCB7EE1A9416A333|scpA]'' mutants are not viable on complex medim that allow rapid growth, but they are viable under conditions of slow growth [Pubmed|24440399,24440393]

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The protein

Protein family

• scpA family (single member, according to UniProt)

Structure

• [PDB|3ZGX] (complex between the head domain of [protein|0136394A52759CEEDD8DE074ED74033C6FFB8435|smc] (aa 1 - 219 and 983 - 1186) and the N-terminal winged-helix domain of [protein|8DD968C97FDC0E81293887E2FCB7EE1A9416A333|scpA]) [Pubmed|23353789]
• [PDB|4I98] (complex between [protein|8DD968C97FDC0E81293887E2FCB7EE1A9416A333|scpA] (aa 1-160)-[protein|9137D162E6331E2B127472BCF5F49A9AE46266F3|scpB] (aa 1-183) [Pubmed|23353789]
• [AF|P35154]

[wiki|Localization]

• nucleoid (Multiple) [Pubmed|16479537]
• the [wiki|condensin] ([protein|0136394A52759CEEDD8DE074ED74033C6FFB8435|smc])₂-[protein|8DD968C97FDC0E81293887E2FCB7EE1A9416A333|scpA]-[protein|9137D162E6331E2B127472BCF5F49A9AE46266F3|scpB] complex forms two bipolar assemblies on the chromosome, one in each cell half, localization depends on [protein|5B3DB5A796ACA48390278E60478DFF13D0074A8D|parA] [Pubmed|24440393,23475963]
• the [wiki|condensin] ([protein|0136394A52759CEEDD8DE074ED74033C6FFB8435|smc])2-[protein|8DD968C97FDC0E81293887E2FCB7EE1A9416A333|scpA]-[protein|9137D162E6331E2B127472BCF5F49A9AE46266F3|scpB] complex is loaded by [protein|EF4BD49FCD49EE97908973581478951C8FA196C0|parB] to parS centromeric sites adjacent to the replication origin, travels then from the origin to the terminus at rates >50 kb per minute [Pubmed|28154080,24440393,23475963]

Biological materials

Mutant

• MGNA-A104 (ypuG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/104 NBRP B. subtilis, Japan]

References

Reviews

• 22934648,22933559,24118085,25460803,26706151,29182912,27075410,34220773

Original Publications

• 12100548,12421306,12065423,7934830,16479537,19450516,15009890,11948165,23353789,23475963,22385855,24440399,24440393,25557547,26253537,25951515,26295962,26725510,26904953,28154080,28286005,28689660,30192981,31201090,31175837,32250245,33472056,33910021,34312537,34346312,35474142