ribU

168

riboflavin [wiki|ECF transporter], S protein

locus: BSU_23050

Molecular weight: 20.41 kDa

pI: 10.12

Protein length: 190 aa

Gene length: 573 bp

function: riboflavin uptake

product: S protein of riboflavin [wiki|ECF transporter]

essential: no

synonyms: ribU, ypaA

List of homologs in different organisms, belongs to COG3601 (Galperin et al., 2021)

Gene

Coordinates: 2,410,017  2,410,589

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The protein

Protein family

• prokaryotic riboflavin transporter (P-RFT) (TC 2.A.87) family (single member, according to UniProt)

Structure

• [PDB|3P5N] (RibU from ''Staphylococcus aureus'')
• [AF|P50726]

[wiki|Localization]

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|651C370386E9FB45E3B98001800D342FEDB43E9A|ribU]

  description: [Pubmed|21815947]

  regulation

  • expressed in the absence of FMN ([protein|search|FMN-box]) [Pubmed|17693491]
  • binding of [protein|3D32B6C1DC9CAC615B02E14EA846648C3B0E61D6|ribR] to the [wiki|FMN-box] riboswitch can enforce expression even in the presence of FMN [pubmed|26494285]

  regulatory mechanism

  • FMN-box: RNA switch, via [protein|C245476CE35028167510F67153F392D91447553F|mgtE riboswitch], sequestration of the Shine-Dalgarno sequence in the presence of FMN or FMNH₂, this is counter-acted upon binding of [protein|3D32B6C1DC9CAC615B02E14EA846648C3B0E61D6|ribR], in [regulon|other_regulator:FMN-box|FMN-box]
  • [protein|3D32B6C1DC9CAC615B02E14EA846648C3B0E61D6|ribR]: antitermination, [pubmed|26494285], in [regulon|protein:3D32B6C1DC9CAC615B02E14EA846648C3B0E61D6|ribR regulon]

  additional information

  • the mRNA is substantially stabilized upon depletion of [gene|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [PubMed|21815947]

Biological materials

Mutant

• MGNA-A478 (ypaA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/478 NBRP B. subtilis, Japan]
• BKE23050 ([gene|651C370386E9FB45E3B98001800D342FEDB43E9A|ribU]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE23050 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACTTCATTCTCTCCTTTTC,  downstream forward: _UP4_TAAATAAAAAAGCTCCCGTT
• BKK23050 ([gene|651C370386E9FB45E3B98001800D342FEDB43E9A|ribU]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK23050 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACTTCATTCTCTCCTTTTC,  downstream forward: _UP4_TAAATAAAAAAGCTCCCGTT

References

Reviews

• 20497229,22574898,24362466

Original Publications

• 12456892,20972419,17693491,21815947,24356467,24755604,25438558,26494285,27312125,17289680,26052893