panC
168
pantothenate synthase
locus
BSU_22420
Molecular weight
31.81 kDa
pI
4.63
function
biosynthesis of coenzyme A
product
pantothenate synthase
essential
no
ec
6.3.2.1
synonyms
panC
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0414 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,352,977 2,353,837
Phenotypes of a mutant
the mutant tends to acquire suppressor mutations that result in improved growth [Pubmed|28189581]
The protein
Catalyzed reaction/ biological activity
(R)-pantoate + ATP + β-alanine --> (R)-pantothenate + AMP + diphosphate + H+ (according to UniProt)
Protein family
pantothenate synthetase family (single member, according to UniProt)
Structure
[PDB|2EJC] (from ''Thermotoga maritima'', 50% identity, 66% similarity)
[AF|P52998]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|A0001681244113B808757281C1B70C0C94F28A27|panB]-[gene|8BE55434677F4EC3D6766C7163496CB1B659F57F|panC]-[gene|EE712C1EC9E9C371847C83F7D75C189B276CD4B6|panD]-[gene|03BFD8AD2E3D95858752F46366F6C2028D709029|dinG]-[gene|08F907C6F47BDEB4DC9A9F136CDC6A4063AC0140|ypmA]-[gene|D3629004F3B54D11566AE15227174F9EB7784201|tseB]-[gene|AE802E1D4F827DED3A5D953C7D034E6A3554C45F|aspB]
description
[Pubmed|22383849]
Biological materials
Mutant
BKE22420 ([gene|8BE55434677F4EC3D6766C7163496CB1B659F57F|panC]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE22420 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TGAAATATCAGTAATCTGTC, downstream forward: _UP4_ATTCGAGAAATGGAGAGAAT
BKK22420 ([gene|8BE55434677F4EC3D6766C7163496CB1B659F57F|panC]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK22420 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TGAAATATCAGTAATCTGTC, downstream forward: _UP4_ATTCGAGAAATGGAGAGAAT
GP3379 ([gene|8BE55434677F4EC3D6766C7163496CB1B659F57F|panC]::neo trpC2), available in [wiki|Jörg Stülke]'s lab, use GP4402
Expression vectors
pGP3723: expression of ''panC'' (''gapA'' RBS) by [wiki|pBQ200] in ''B. subtilis'', available in [wiki|Jörg Stülke]'s lab
References
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Time of last update: 2024-03-24 17:58:24
Author of last update: Christoph.elfmann