desK

168

two-component sensor kinase, regulation of cold shock expression of [gene|644C0C354B72FC07222EE45F4D2E0E57434B5EB5|des]

locus: BSU_19190

Molecular weight: 42.51 kDa

pI: 9.43

Protein length: 370 aa

Gene length: 1113 bp

function: regulation of cold shock expression of [gene|644C0C354B72FC07222EE45F4D2E0E57434B5EB5|des]

product: two-component sensor kinase

essential: no

synonyms: desK, yocF

List of homologs in different organisms, belongs to COG4585 (Galperin et al., 2021)

Gene

Coordinates: 2,090,574 → 2,091,686

Advertisement

The protein

Catalyzed reaction/ biological activity

• autophosphorylation, phosphorylation of [protein|F08BE4ACC0ECE1416B012CA72B027423D4D9B00A|desR]
• ATP + [protein|C5F9A1970393625B01C05C72C9A240C8EE8789D3|desK] L-histidine --> ADP + [protein|C5F9A1970393625B01C05C72C9A240C8EE8789D3|desK]-N-phospho-L-histidine (according to UniProt)

[wiki|Domains]

• 5 transmembrane helices
• cytoplasmatic C-terminal trail
• [wiki|Histidine kinase domain] (aa 186-369) (according to UniProt)

[wiki|Cofactors]

• Mg²⁺ [pubmed|36693130]

Structure

• [PDB|3EHF] [Pubmed|19805278]
• [PDB|5IUJ]([protein|C5F9A1970393625B01C05C72C9A240C8EE8789D3|desK]-[protein|F08BE4ACC0ECE1416B012CA72B027423D4D9B00A|desR] complex in the phosphotransfer state with low Mg2+ [20 mM]) [Pubmed|27938660]
• [PDB|5IUK]([protein|C5F9A1970393625B01C05C72C9A240C8EE8789D3|desK]-[protein|F08BE4ACC0ECE1416B012CA72B027423D4D9B00A|desR] complex in the phosphotransfer state with high Mg2+ [150 mM] and BeF3) [Pubmed|27938660]
• [PDB|5IUM](phosphorylated wild type [protein|C5F9A1970393625B01C05C72C9A240C8EE8789D3|desK]C) [Pubmed|27938660]
• [PDB|5IUN]([protein|C5F9A1970393625B01C05C72C9A240C8EE8789D3|desK]-[protein|F08BE4ACC0ECE1416B012CA72B027423D4D9B00A|desR] complex in the phosphatase state) [Pubmed|27938660]
• [AF|O34757]

Modification

• autophosphorylation on a His residue

Effectors of protein activity

• unsaturated fatty acids are negative effectors of the system
• acidic pH results in protonation of glutamate residues and breaking of intramolecular salt bridges, this results in helix destabilization, [protein|F08BE4ACC0ECE1416B012CA72B027423D4D9B00A|desR] will not be activated [pubmed|32823946]

Paralogous protein(s)

• [protein|91110611D775A1746766D43BDF50F2F6D345B625|yvfT]

[wiki|Localization]

• membrane (transmembrane segments)

Additional information

• [protein|C5F9A1970393625B01C05C72C9A240C8EE8789D3|desK] has the ability to sense changes in membrane fluidity [Pubmed|17087771]

Expression and Regulation

Operons

• genes: [gene|C5F9A1970393625B01C05C72C9A240C8EE8789D3|desK]-[gene|F08BE4ACC0ECE1416B012CA72B027423D4D9B00A|desR]

  description: [Pubmed|11285232]

  regulation

  • induced by cold shock (12-fold) [Pubmed|12399512]

Biological materials

Mutant

• MGNA-A327 (yocF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/327 NBRP B. subtilis, Japan]
• BKE19190 (Δ[gene|C5F9A1970393625B01C05C72C9A240C8EE8789D3|desK]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE19190 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGATCTTACCTCATTTTC,  downstream forward: _UP4_AAATAAACATAAAGGATGGC
• BKK19190 (Δ[gene|C5F9A1970393625B01C05C72C9A240C8EE8789D3|desK]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK19190 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGATCTTACCTCATTTTC,  downstream forward: _UP4_AAATAAACATAAAGGATGGC

labs

• [wiki|Diego de Mendoza], Universidad Nacional de Rosario, Argentine [http://www.ibr.gov.ar/eng/investigacion/demendoza.htm homepage]

References

Reviews

• 20117042,17087771,24819366

Original Publications

• 10094672,14734164,12399512,20507988,19233289,11285232,19805278,15090506,12207704,20705470,23356219,24574048,24522108,25406381,26172072,27528507,27938660,28475313,29269314,30431418,31772055,32823946,34201916,36693130