fosB

168

bacillithiol-S-transferase; confers resistence against antimicrobial compounds from B. amyloliquefaciens

locus: BSU_17840

Molecular weight: 17.03 kDa

pI: 6.14

Protein length: 144 aa

Gene length: 435 bp

function: confers resistence against antimicrobial compounds from B. amyloliquefaciens

product: fosfomycin resistance protein

essential: no

synonyms: fosB, yndN

List of homologs in different organisms, belongs to COG0346 (Galperin et al., 2021)

Gene

Coordinates: 1,916,663  1,917,097

Phenotypes of a mutant

• sensitive to fosfomycin [pubmed|11244082]

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The protein

Protein family

• fosfomycin resistance protein family (single member, according to UniProt)

[wiki|Domains]

• [wiki|VOC domain] (aa 5-120) (according to UniProt)

[wiki|Cofactors]

• Mg²⁺ [pubmed|11244082]

Structure

• [PDB|4JD1] (from B. anthracis, 66% identity)
• [AF|O31817]

[wiki|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|92E4F908B7CDE64743796CCD3188FE6BB5288365|fosB]

  description: [Pubmed|11244082]

  regulation

  • expressed under stress conditions ([protein|search|SigW]) [Pubmed|11244082]

  sigma factors

  • [protein|3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|sigW]: sigma factor, [Pubmed|11244082], in [regulon|protein:3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|sigW regulon]

  additional information

  • A [protein|search|ncRNA] is predicted between '[protein|search|fosB]' and '[protein|search|lexA]' [PubMed|20525796]

Biological materials

Mutant

• MGNA-B387 (yndN::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1386 NBRP B. subtilis, Japan]
• BKE17840 ([gene|92E4F908B7CDE64743796CCD3188FE6BB5288365|fosB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE17840 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACAAATCATTCCCCCCTTG,  downstream forward: _UP4_TGATAGCACAACCATATTTC
• BKK17840 ([gene|92E4F908B7CDE64743796CCD3188FE6BB5288365|fosB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK17840 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACAAATCATTCCCCCCTTG,  downstream forward: _UP4_TGATAGCACAACCATATTTC

References

• 11244082,16629676,20525796,23030527,23256780