xynP

168

beta-xyloside permease

locus: BSU_17570

Molecular weight: 51.37 kDa

pI: 8.58

Protein length: 463 aa

Gene length: 1392 bp

function: xylan utilization

product: beta-xyloside permease

essential: no

synonyms: xynP, ynaJ

List of homologs in different organisms, belongs to COG2211 (Galperin et al., 2021)

Gene

Coordinates: 1,887,352 → 1,888,743

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The protein

Protein family

• [wiki|sodium:galactoside symporter (TC 2.A.2) family] (according to UniProt)

Structure

• [PDB|4M64] (melibiose permease from Salmonella typhimurium, 31% identity) [pubmed|24389923]
• [AF|P94488]

Paralogous protein(s)

• [protein|B13F7C35FBD55C674E5B19836D32279E7C1A5520|gutP], [protein|59CD3E4C24326542D9C85BB6FE64FBF49869EB55|yjmB]

[wiki|Localization]

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|F92925E2FDCFE6DDA3E87C7B541FE6A6CE5AABB8|xynP]-[gene|19CD9BF216953613CD2E0DFB99D4C6E92AAFAE5D|xynB]

  description: [Pubmed|22383849]

  regulation

  • carbon catabolite repression ([protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]) [Pubmed|9973552]
  • induction by xylose ([protein|AF4395D134485290F4AA307B48494FED39E52CD7|xylR]) [Pubmed|9973552]

  regulatory mechanism

  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: repression, [Pubmed|9973552], in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]
  • [protein|AF4395D134485290F4AA307B48494FED39E52CD7|xylR]: repression, [Pubmed|9973552], in [regulon|protein:AF4395D134485290F4AA307B48494FED39E52CD7|xylR regulon]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|9973552], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]

Biological materials

Mutant

• MGNA-B107 (ynaJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1106 NBRP B. subtilis, Japan]
• BKE17570 (Δ[gene|F92925E2FDCFE6DDA3E87C7B541FE6A6CE5AABB8|xynP]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE17570 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGATTTCTCCCCTTTTCG,  downstream forward: _UP4_TAAAAAAGAAAATAAACTGA
• BKK17570 (Δ[gene|F92925E2FDCFE6DDA3E87C7B541FE6A6CE5AABB8|xynP]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK17570 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGATTTCTCCCCTTTTCG,  downstream forward: _UP4_TAAAAAAGAAAATAAACTGA

lacZ fusion

• pGP417 (in [wiki|pAC7]), available in [wiki|Jörg Stülke]'s lab

References

Carbon catabolite repression

• 18757537,9973552,22001508,22900538,24389923