pksG

168

involved in polyketide synthesis

locus: BSU_17150

Molecular weight: 46.62 kDa

pI: 5.14

Protein length: 420 aa

Gene length: 1263 bp

function: polyketide synthesis

product: hydroxymethylglutaryl CoA synthase

essential: no

synonyms: pksG

List of homologs in different organisms, belongs to COG3425 (Galperin et al., 2021)

Gene

Coordinates: 1,789,943 → 1,791,205

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The protein

Catalyzed reaction/ biological activity

• 3-oxobutanoyl-[ACP] + acetyl-[ACP] + H₂O --> (3S)-hydroxy-3-methylglutaryl-[ACP] + H⁺ + holo-[ACP] (according to UniProt)

Protein family

• [wiki|thiolase-like superfamily] (according to UniProt)

Structure

• [PDB|4YXQ]
• [PDB|4YXT] (E82A mutant)
• [PDB|4YXV] (C114A mutant)
• [AF|P40830]

Biological materials

Mutant

• MGNA-A017 (pksG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/17 NBRP B. subtilis, Japan]
• BKE17150 (Δ[gene|F4C96980640CB04D3017B8EBB02918006CBCF7BB|pksG]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE17150 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CGCTTCTATTCCGGCTGAAA,  downstream forward: _UP4_TCTGAGTTTCATCGGAAGTA
• BKK17150 (Δ[gene|F4C96980640CB04D3017B8EBB02918006CBCF7BB|pksG]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK17150 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CGCTTCTATTCCGGCTGAAA,  downstream forward: _UP4_TCTGAGTTTCATCGGAAGTA

References

• 23840410,22383849,24187085,27766092